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粪便标本中检测十二指肠贾第鞭毛虫的DNA提取和PCR方法的敏感性评估。

Evaluation of the sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens.

作者信息

Nantavisai Kwannan, Mungthin Mathirut, Tan-ariya Peerapan, Rangsin Ram, Naaglor Tawee, Leelayoova Saovanee

机构信息

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

出版信息

J Clin Microbiol. 2007 Feb;45(2):581-3. doi: 10.1128/JCM.01823-06. Epub 2006 Nov 22.

DOI:10.1128/JCM.01823-06
PMID:17122010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1829074/
Abstract

Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitivities and specificities.

摘要

对用于十二指肠贾第虫的DNA提取方法和PCR方法的敏感性进行了评估。最敏感的方法组合，即FTA滤纸片和使用RH11/RH4及GiarF/GiarR引物的PCR方案，在敏感性和特异性方面与免疫荧光分析相比没有显著差异。

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Vet Parasitol. 2005 Jun 30;130(3-4):177-83. doi: 10.1016/j.vetpar.2005.03.032. Epub 2005 Apr 25.
2
Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP.利用聚合酶链反应-限制性片段长度多态性技术对十二指肠贾第鞭毛虫谷氨酸脱氢酶基因座的所有基因型进行鉴别。
Infect Genet Evol. 2004 Jun;4(2):125-30. doi: 10.1016/j.meegid.2004.02.001.
3
Epidemiological and molecular evidence supports the zoonotic transmission of Giardia among humans and dogs living in the same community.流行病学和分子证据支持贾第虫在同一社区生活的人类和狗之间的人畜共患传播。
Parasitology. 2004 Mar;128(Pt 3):253-62. doi: 10.1017/s0031182003004505.
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Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis.磷酸丙糖异构酶基因特征及十二指肠贾第虫潜在的人畜共患病传播
Emerg Infect Dis. 2003 Nov;9(11):1444-52. doi: 10.3201/eid0911.030084.
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Evaluation of three commercial assays for detection of Giardia and Cryptosporidium organisms in fecal specimens.评估三种用于检测粪便标本中贾第虫和隐孢子虫的商业检测方法。
J Clin Microbiol. 2003 Feb;41(2):623-6. doi: 10.1128/JCM.41.2.623-626.2003.
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Current trends in research into the waterborne parasite Giardia.水源性寄生虫贾第虫的当前研究趋势
Crit Rev Microbiol. 2002;28(2):123-47. doi: 10.1080/1040-840291046713.
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Int J Parasitol. 2002 Jul;32(8):1023-30. doi: 10.1016/s0020-7519(02)00068-1.
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Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa.用于快速灵敏地PCR检测致病性寄生原生动物的免提取、基于过滤的模板制备方法。
J Clin Microbiol. 2000 Jun;38(6):2271-7. doi: 10.1128/JCM.38.6.2271-2277.2000.
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