Clarke G M, Eidt S, Sun L, Mawdsley G, Zubovits J T, Yaffe M J
Imaging Research, Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada.
Histopathology. 2007 Jan;50(2):232-42. doi: 10.1111/j.1365-2559.2006.02561.x.
To develop a method for preparing diagnostic-quality, whole-mount serial sections of breast specimens while preserving 3-D conformation. This required supporting the fresh specimen prior to breadloafing and refining the conventional tissue processing method. The overall goal is to use digital images of whole-specimen histopathology to improve the estimation of extent of disease.
To maintain a 3-D conformation, the specimen is suspended in 3.5% agar at 55 degrees C. The block is sliced at 5-mm intervals. Sectioning is performed after extended fixation in 4% formaldehyde from paraformaldehyde in 0.1 m Millonig's buffer, followed by paraffin processing using a non-routine schedule and extended paraffin infiltration. Whole-mount serial breast sections are produced with features of equal or superior quality to that which can be achieved using conventional methods. The method is compatible with some immunohistochemical stains but requires further optimization for others.
The technique is currently suitable for research applications. With the reduction in processing time achievable with microwave-assisted processing, there is the potential for its use as a routine clinical method. This tool may improve the accuracy of margin estimates and identification of multifocality in breast cancer; further evaluation is necessary.
开发一种在保留三维构象的同时制备乳腺标本诊断质量全层连续切片的方法。这需要在制作面包条样标本之前对新鲜标本进行支撑，并改进传统的组织处理方法。总体目标是利用全标本组织病理学的数字图像来改进疾病范围的估计。
为保持三维构象，将标本悬浮于55℃的3.5%琼脂中。以5毫米的间隔对组织块进行切片。在0.1m米隆尼格缓冲液中用多聚甲醛配制的4%甲醛进行长时间固定后进行切片，然后采用非常规流程进行石蜡处理并延长石蜡浸润时间。制作出的全层连续乳腺切片具有与传统方法相当或更优的质量特征。该方法与某些免疫组织化学染色兼容，但其他染色则需要进一步优化。
该技术目前适用于研究应用。随着微波辅助处理可实现处理时间的缩短，它有可能用作常规临床方法。该工具可能提高乳腺癌切缘估计和多灶性识别的准确性；有必要进行进一步评估。
