Osorio Edison, Arango Gabriel Jaime, Jiménez Nora, Alzate Fernando, Ruiz Grace, Gutiérrez David, Paco Marco Antonio, Giménez Alberto, Robledo Sara
Grupo de Investigación en Sustancias Bioactivas (GISB), Facultad de Química Farmacéutica, Corporación de Patologías Tropicales, Universidad de Antioquia, A.A. 1226, Medellín, Colombia.
J Ethnopharmacol. 2007 May 22;111(3):630-5. doi: 10.1016/j.jep.2007.01.015. Epub 2007 Jan 18.
Ethnobotanical and chemotaxonomical studies for antiparasitic activity of Colombian Annonaceae were carried out. In vitro antiprotozoal activity of 36 extracts obtained from six different species was determined against promastigotes of three Leishmania species, epimastigotes of Trypanosoma cruzi and both chloroquine sensitive (F32) and resistant (W2) Plasmodium falciparum. Cytotoxic activity was evaluated in U-937 cells. Active extracts were selected according their selectivity index (SI). Extracts from Annona muricata, Rollinia exsucca, Rollinia pittieri and Xylopia aromatica were active against Leishmania spp. and Trypanosoma cruzi showing IC50 values lower than 25 microg/ml. Hexane extract from Rollinia pittieri leaves was the most selective against Trypanosoma cruzi and Leishmania spp. (IS=10 and 16, respectively). The extracts from Desmopsis panamensis, Pseudomalmea boyacana, Rollinia exsucca and Rollinia pittieri showed good antiplasmodial activity (IC50 < 10 microg/ml). No correlation between antiplasmodial activity and inhibition of beta-hematin production was found. The present study gives specific and useful information about antiprotozoal and cytotoxic activities of some Annonaceae extracts. Results presented here also demonstrate which plants and/or plant parts could be useful in the treatment of leishmaniasis, Chagas' disease and malaria.
开展了关于哥伦比亚番荔枝科植物抗寄生虫活性的民族植物学和化学分类学研究。测定了从六个不同物种中获得的36种提取物对三种利什曼原虫前鞭毛体、克氏锥虫上鞭毛体以及氯喹敏感（F32）和耐药（W2）恶性疟原虫的体外抗原生动物活性。在U - 937细胞中评估细胞毒性活性。根据其选择性指数（SI）选择活性提取物。刺果番荔枝、苏卡番荔枝、皮氏番荔枝和芳香团香果的提取物对利什曼原虫属和克氏锥虫具有活性，其半数抑制浓度（IC50）值低于25微克/毫升。皮氏番荔枝叶的己烷提取物对克氏锥虫和利什曼原虫属的选择性最高（选择性指数分别为10和16）。巴拿马地萼木、博亚卡拟单性木兰、苏卡番荔枝和皮氏番荔枝的提取物显示出良好的抗疟活性（IC50 < 10微克/毫升）。未发现抗疟活性与β-血红素生成抑制之间的相关性。本研究提供了关于一些番荔枝科植物提取物的抗原生动物和细胞毒性活性的具体且有用的信息。这里呈现的结果还表明哪些植物和/或植物部位可用于治疗利什曼病、恰加斯病和疟疾。
