Romano Ida, Lama Licia, Nicolaus Barbara, Poli Annarita, Gambacorta Agata, Giordano Assunta
Istituto di Chimica Biomolecolare, Comprensorio ex Olivetti, Pozzouli, Na, Italy.
J Gen Appl Microbiol. 2006 Dec;52(6):339-48. doi: 10.2323/jgam.52.339.
A halotolerant and alkaliphilic Gram-negative bacterium, strain 18bAG(T), that grows aerobically at the optimum temperature of 37 degrees C, and at pH 7.5-10 (optimum 9.0), was isolated from a salt pool located in Montefredane in Campania Region (South of Italy). The isolate tolerated high concentration of NaCl up to 20%. Strain 18bAG(T) accumulated osmolytes and polyhydroxybutyrate, produced exopolysaccharide and possessed alpha-glucosidase activity. The predominant respiratory quinones were ubiquinones, Q8 and Q6(6H); phosphoethanolamine, phosphatidylglycerol and diphosphatidylglycerol were the predominant polar lipids. Major fatty acids were C16 : 1, C16 : 0, and C18 : 0. On the basis of 16S rRNA gene sequence similarity, 18bAG(T) was shown to belong to Halomonas genus. Analysis of 16S rRNA gene revealed a high similarity of strain 18bAG(T) to Halomonas venusta (DSM 4743(T)) and Halomonas hydrothermalis (DSM 15725(T)). Level of DNA-DNA relatedness between strain 18bAG(T) and the most related species Halomonas venusta and Halomonas hydrothermalis was 56.0% and 41.2%, respectively. The G+C content (mol%) of DNA was 53.0. The RiboPrinting patterns of Halomonas venusta and 18AG(T) showed a pattern similarity of 0.50. On the basis of genomic information and phenotypic characteristics strain 18bAG(T) represents a new species, for which the name Halomonas alkaliphila sp. nov. is proposed. The type strain is 18bAG(T) (=DSM 16354T =ATCC BAA-953T).
从意大利南部坎帕尼亚地区蒙特弗雷达内的一个盐池中分离出一株耐盐嗜碱革兰氏阴性菌，菌株18bAG(T)。该菌株在37℃的最适温度以及pH 7.5 - 10（最适pH 9.0）条件下好氧生长。该分离株能耐受高达20%的高浓度氯化钠。菌株18bAG(T)积累渗透保护剂和聚羟基丁酸酯，产生胞外多糖并具有α-葡萄糖苷酶活性。主要呼吸醌为泛醌Q8和Q6(6H)；磷酸乙醇胺、磷脂酰甘油和双磷脂酰甘油是主要极性脂质。主要脂肪酸为C16 : 1、C16 : 0和C18 : 0。基于16S rRNA基因序列相似性，18bAG(T)被证明属于嗜盐单胞菌属。16S rRNA基因分析显示菌株18bAG(T)与盐沼嗜盐单胞菌（DSM 4743(T)）和热液嗜盐单胞菌（DSM 15725(T)）高度相似。菌株18bAG(T)与最相关物种盐沼嗜盐单胞菌和热液嗜盐单胞菌之间的DNA - DNA相关性水平分别为56.0%和41.2%。DNA的G + C含量（mol%）为53.0。盐沼嗜盐单胞菌和18AG(T)的核糖体印记图谱显示模式相似性为0.50。基于基因组信息和表型特征，菌株18bAG(T)代表一个新物种，为此提出新名称嗜碱嗜盐单胞菌（Halomonas alkaliphila sp. nov.）。模式菌株为18bAG(T)（=DSM 16354T =ATCC BAA - 953T）。
