Méndez-Moreno Evelyn, Caporal-Hernandez Liliana, Mendez-Pfeiffer Pablo A, Enciso-Martinez Yessica, De la Rosa López Rafael, Valencia Dora, Arenas-Hernández Margarita M P, Ballesteros-Monrreal Manuel G, Barrios-Villa Edwin
Departamento de Ciencias Químico Biológicas y Agropecuarias, Universidad de Sonora, Unidad Regional Norte, Campus Caborca. Av. Universidad e Irigoyen S/N, col. Eleazar Ortiz, H. Caborca CP 83621, Sonora, Mexico.
Posgrado en Microbiología, Centro de Investigación en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Ciudad Universitaria, Puebla CP 72570, Pue, Mexico.
Antibiotics (Basel). 2022 Jun 21;11(7):833. doi: 10.3390/antibiotics11070833.
is a well-recognized inhabitant of the animal and human gut. Its presence represents an essential component of the microbiome. There are six pathogenic variants of associated with diarrheal processes, known as pathotypes. These harbor genetic determinants that allow them to be classified as such. In this work, we report the presence of diarrheagenic pathotypes of strains isolated from healthy donors. Ninety strains were analyzed, of which forty-six (51%) harbored virulence markers specifics for diarrheagenic pathotypes, including four hybrids (one of them with genetic determinants of three DEC pathotypes). We also identified phylogenetic groups with a higher prevalence of B2 (45.6%) and A (17.8%). In addition, resistance to sulfonamides (100%), and aminoglycosides (100%) was found in 100% of the strains, with a lower prevalence of resistance to cefotaxime (13.3%), ceftriaxone (12.2%), fosfomycin (10%), and meropenem (0%). All analyzed strains were classified as multidrug resistant. Virulence genes were also investigated, which led us to propose three new virotypes. Among the virulence traits observed, the ability to form biofilms stands out, which was superior to that of the and strains used as positive controls.
是动物和人类肠道中广为人知的居民。它的存在是微生物群的重要组成部分。有六种与腹泻过程相关的致病变体，称为致病型。这些变体具有使其能够被如此分类的遗传决定因素。在这项工作中，我们报告了从健康供体分离的菌株中腹泻致病型的存在。分析了90株菌株，其中46株（51%）携带腹泻致病型特有的毒力标记，包括4株杂交株（其中1株具有三种腹泻大肠埃希菌致病型的遗传决定因素）。我们还确定了B2（45.6%）和A（17.8%）患病率较高的系统发育组。此外，在100%的菌株中发现对磺胺类药物（100%）和氨基糖苷类药物（100%）耐药，对头孢噻肟（13.3%）、头孢曲松（12.2%）、磷霉素（10%）和美罗培南（0%）耐药的患病率较低。所有分析的菌株都被归类为多重耐药。还对毒力基因进行了研究，这使我们提出了三种新的病毒型。在所观察到的毒力特征中，可以形成生物膜的能力最为突出，其优于用作阳性对照的菌株和菌株。
