Kaigala Govind V, Ho Sunny, Penterman Roel, Backhouse Christopher J
Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, CanadaT6G 2V4.
Lab Chip. 2007 Mar;7(3):384-7. doi: 10.1039/b617764f. Epub 2007 Jan 10.
We demonstrate a rapid and inexpensive approach for the fabrication of high resolution poly(dimethylsiloxane) (PDMS)-based microfluidic devices. The complete process of fabrication could be performed in several hours (or less) without any specialized equipment other than a consumer-grade wax printer. The channels produced by this method are of high enough quality that we are able to demonstrate the sizing and separation of DNA fragments using capillary electrophoresis (CE) with no apparent loss of resolution over that found with glass chips fabricated by conventional photolithographic methods. We believe that this method will greatly improve the accessibility of rapid prototyping methods.
我们展示了一种快速且经济的方法来制造基于高分辨率聚二甲基硅氧烷（PDMS）的微流控装置。除了消费级蜡打印机外，无需任何专门设备，整个制造过程可在数小时（或更短时间）内完成。通过这种方法制造的通道质量足够高，以至于我们能够使用毛细管电泳（CE）来展示DNA片段的大小测定和分离，与传统光刻方法制造的玻璃芯片相比，分辨率没有明显损失。我们相信这种方法将大大提高快速原型制作方法的可及性。
