Smith Joshua E, Medley Colin D, Tang Zhiwen, Shangguan Dihua, Lofton Charles, Tan Weihong
Center for Research at the Bio/Nano Interface, Department of Chemistry, University of Florida, Gainesville, Florida 32611, USA.
Anal Chem. 2007 Apr 15;79(8):3075-82. doi: 10.1021/ac062151b. Epub 2007 Mar 10.
We have extended the use the aptamer-conjugated nanoparticles for the collection and detection of multiple cancer cells. The aptamers were selected using a cell-based SELEX strategy in our laboratory for cancer cells that, when utilized in this method, allow for the selective recognition of the cells from complex mixtures including fetal bovine serum samples. Aptamer-conjugated magnetic nanoparticles were used for the selective targeting cell extraction, and aptamer-conjugated fluorescent nanoparticles were employed for sensitive cellular detection. Employing both types of nanoparticles allows for selective and sensitive detection not possible by using the particles separately. Fluorescent nanoparticles amplify the signal intensity versus a single fluorophore label resulting in improved sensitivity. In addition, aptamer-conjugated magnetic nanoparticles allow for extraction and enrichment of target cells not possible with other separation methods. Fluorescent imaging and a microplate reader were used for cellular detection to demonstrate the wide applicability of this methodology for medical diagnostics and cell enrichment and separation.
我们已经扩展了适体缀合纳米颗粒在多种癌细胞收集和检测方面的应用。这些适体是我们实验室采用基于细胞的SELEX策略针对癌细胞筛选出来的，当用于此方法时，能够从包括胎牛血清样本在内的复杂混合物中选择性识别细胞。适体缀合磁性纳米颗粒用于选择性靶向细胞提取，适体缀合荧光纳米颗粒用于灵敏的细胞检测。同时使用这两种纳米颗粒可实现单独使用颗粒时无法达到的选择性和灵敏检测。与单个荧光团标记相比，荧光纳米颗粒可放大信号强度，从而提高灵敏度。此外，适体缀合磁性纳米颗粒能够实现其他分离方法无法完成的靶细胞提取和富集。利用荧光成像和微孔板读数器进行细胞检测，以证明该方法在医学诊断以及细胞富集和分离方面具有广泛的适用性。
