Bryant Stephanie J, Cuy Janet L, Hauch Kip D, Ratner Buddy D
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA.
Biomaterials. 2007 Jul;28(19):2978-86. doi: 10.1016/j.biomaterials.2006.11.033. Epub 2007 Mar 29.
Since pore size and geometry strongly impact cell behavior and in vivo reaction, the ability to create scaffolds with a wide range of pore geometries that can be tailored to suit a particular cell type addresses a key need in tissue engineering. In this contribution, we describe a novel and simple technique to design porous, degradable poly(2-hydroxyethyl methacrylate) hydrogel scaffolds with well-defined architectures using a unique photolithography process and optimized polymer chemistry. A sphere-template was used to produce a highly uniform, monodisperse porous structure. To create a patterned and porous hydrogel scaffold, a photomask and initiating light were employed. Open, vertical channels ranging in size from 360+/-25 to 730+/-70 microm were patterned into approximately 700 microm thick hydrogels with pore diameters of 62+/-8 or 147+/-15 microm. Collagen type I was immobilized onto the scaffolds to facilitate cell adhesion. To assess the potential of these novel scaffolds for tissue engineering, a skeletal myoblast cell line (C2C12) was seeded onto scaffolds with 147 microm pores and 730 microm diameter channels, and analyzed by histology and digital volumetric imaging. Cell elongation, cell spreading and fibrillar formation were observed on these novel scaffolds. In summary, 3D architectures can be patterned into porous hydrogels in one step to create a wide range of tissue engineering scaffolds that may be tailored for specific applications.
由于孔径和几何形状会强烈影响细胞行为和体内反应，因此能够制造出具有多种可定制以适应特定细胞类型的孔几何形状的支架，满足了组织工程中的一项关键需求。在本论文中，我们描述了一种新颖且简单的技术，使用独特的光刻工艺和优化的聚合物化学方法，设计具有明确结构的多孔、可降解聚（甲基丙烯酸2-羟乙酯）水凝胶支架。使用球形模板来制备高度均匀、单分散的多孔结构。为了制造有图案的多孔水凝胶支架，采用了光掩模和引发光。在约700微米厚、孔径为62±8或147±15微米的水凝胶中，形成了尺寸范围从360±25到730±70微米的开放垂直通道图案。将I型胶原蛋白固定在支架上以促进细胞黏附。为了评估这些新型支架在组织工程中的潜力，将骨骼肌成肌细胞系（C2C12）接种到具有147微米孔径和730微米直径通道的支架上，并通过组织学和数字体积成像进行分析。在这些新型支架上观察到了细胞伸长、细胞铺展和纤维形成。总之，可以一步将三维结构图案化到多孔水凝胶中，以制造出一系列可为特定应用量身定制的组织工程支架。
