Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A S, Grant Maria B, Lanza Robert
Advanced Cell Technology, Worcester, Massachusetts 01605, USA.
Nat Methods. 2007 Jun;4(6):501-9. doi: 10.1038/nmeth1041. Epub 2007 May 7.
Recent evidence suggests the existence of progenitor cells in adult tissues that are capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Here we describe an efficient and reproducible method for generating large numbers of these bipotential progenitors-known as hemangioblasts-from human embryonic stem (hES) cells using an in vitro differentiation system. Blast cells expressed gene signatures characteristic of hemangioblasts, and could be expanded, cryopreserved and differentiated into multiple hematopoietic lineages as well as into endothelial cells. When we injected these cells into rats with diabetes or into mice with ischemia-reperfusion injury of the retina, they localized to the site of injury in the damaged vasculature and appeared to participate in repair. Injection of the cells also reduced the mortality rate after myocardial infarction and restored blood flow in hind limb ischemia in mouse models. Our data suggest that hES-derived blast cells (hES-BCs) could be important in vascular repair.
近期证据表明，成体组织中存在祖细胞，它们能够分化为血管结构以及所有造血细胞谱系。在此，我们描述了一种高效且可重复的方法，利用体外分化系统从人胚胎干细胞（hES细胞）中生成大量这类双能祖细胞——即所谓的成血管细胞。胚细胞表达了成血管细胞特有的基因特征，并且能够扩增、冻存并分化为多种造血谱系以及内皮细胞。当我们将这些细胞注射到患有糖尿病的大鼠或患有视网膜缺血再灌注损伤的小鼠体内时，它们定位于受损脉管系统的损伤部位，并似乎参与了修复过程。在小鼠模型中，注射这些细胞还降低了心肌梗死后的死亡率，并恢复了后肢缺血中的血流。我们的数据表明，hES衍生的胚细胞（hES-BCs）在血管修复中可能具有重要作用。
