Koskinen Walter J, Brøndbo Kjell, Mellin Dahlstrand Hanna, Luostarinen Tapio, Hakulinen Timo, Leivo Ilmo, Molijn Anco, Quint Wim G, Røysland Tov, Munck-Wikland Eva, Mäkitie Antti A, Pyykkö Ilmari, Dillner Joakim, Vaheri Antti, Aaltonen Leena-Maija
Department of Virology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, P.O. Box 21, 00014 Helsinki, Finland.
J Cancer Res Clin Oncol. 2007 Sep;133(9):673-8. doi: 10.1007/s00432-007-0219-8. Epub 2007 May 8.
Human papillomavirus (HPV) has been linked to oropharyngeal carcinomas, but its role in laryngeal squamous cell carcinoma (LSCC) is not clear. A prospective multicenter study based on known tumor-cell percentage of fresh frozen carcinoma biopsies was established to determine the HPV prevalence. Moreover risk factors such as smoking, alcohol abuse, chronic laryngitis and gastroesophageal reflux disease (GERD) were evaluated
Fresh-frozen laryngeal cancer biopsies from 108 patients in Finland, Norway, and Sweden were investigated. Patients whose biopsy samples contained at least 20% tumor tissue (N = 69) entered the study. HPV DNA was determined with MY09/11 and GP5+/6+ nested PCR and SPF10 PCR hybridization assay. Patients were examined by an ENT specialist and an extensive questionnaire concerning risk factors was filled in.
Only three patients (4.4%) harbored HPV DNA in their carcinoma sample. Heavy alcohol drinking was associated with an increased risk of death, advanced-stage disease, and younger age at diagnosis. Chronic laryngitis, GERD, and orogenital sex contacts were rare. Poor oral hygiene was not associated with survival, although it correlated with heavy drinking.
In our series HPV was not important in LSCC. Heavy drinking led to major mortality in LSCC and promoted early carcinogenesis.
人乳头瘤病毒（HPV）与口咽癌有关，但其在喉鳞状细胞癌（LSCC）中的作用尚不清楚。本研究建立了一项基于已知新鲜冷冻癌活检组织肿瘤细胞百分比的前瞻性多中心研究，以确定HPV的患病率。此外，还评估了吸烟、酗酒、慢性喉炎和胃食管反流病（GERD）等危险因素。
对来自芬兰、挪威和瑞典的108例患者的新鲜冷冻喉癌活检组织进行研究。活检样本中肿瘤组织至少占20%的患者（N = 69）进入研究。采用MY09/11和GP5+/6+巢式PCR以及SPF10 PCR杂交检测法测定HPV DNA。由耳鼻喉科专家对患者进行检查，并填写一份关于危险因素的详细问卷。
只有3例患者（4.4%）的癌样本中检测到HPV DNA。大量饮酒与死亡风险增加、疾病晚期以及诊断时年龄较轻有关。慢性喉炎、GERD和口交性行为较为罕见。口腔卫生差与生存率无关，尽管它与大量饮酒有关。
在我们的研究系列中，HPV在LSCC中并不重要。大量饮酒导致LSCC的主要死亡，并促进早期癌变。
