Stender Rachell N, Engler Whitney Jp, Braun Thomas M, Hankenson F Claire
Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI, USA.
J Am Assoc Lab Anim Sci. 2007 May;46(3):47-52.
Portable clinical analyzers are currently used in human and veterinary medicine for diagnostic testing and blood monitoring; however, normal values for mice and rats of varying genetic backgrounds have not previously been reported. Blood was collected from unanesthetized mice (n = 131) and rats (n = 76) into lithium heparin tubes for analysis using E6+ cartridges for the portable analyzer. Results of glucose, blood urea nitrogen (BUN), sodium, potassium, chloride, hematocrit, and hemoglobin were compared to published ranges provided by a contract diagnostic laboratory. Analyzer ranges were computed as the mean +/- 2 standard deviations of the test samples, such that approximately 95% of tested animals would fall within the resultant range. The degree of overlap between analyzer and published ranges, or the percentage of the published range contained within the analyzer range, was calculated for all analytes. For mice, the ranges of 5 of 7 analytes had more than 57% overlap; for rats, ranges for 6 of 7 analytes had over 65% overlap. After the establishment of normal ranges, the analyzer was used to confirm hyperglycemia in Type I diabetic mice and elevated BUN in rats with induced glomerulosclerosis. The portable analyzer can be a valuable screening tool for both phenotyping and clinical care of rodents, with potential for investigations of both spontaneous and experimental disease in laboratory rodents.
便携式临床分析仪目前用于人类和兽医学的诊断测试和血液监测；然而，此前尚未报道过不同遗传背景的小鼠和大鼠的正常数值。将未麻醉的小鼠（n = 131）和大鼠（n = 76）的血液采集到含锂肝素的试管中，使用便携式分析仪的E6 + 检测卡进行分析。将葡萄糖、血尿素氮（BUN）、钠、钾、氯、血细胞比容和血红蛋白的检测结果与一家合同诊断实验室提供的已发表范围进行比较。分析仪范围计算为测试样本的平均值±2个标准差，这样大约95%的测试动物将落在所得范围内。计算所有分析物的分析仪范围与已发表范围之间的重叠程度，或已发表范围包含在分析仪范围内的百分比。对于小鼠，7种分析物中的5种范围有超过57%的重叠；对于大鼠，7种分析物中的6种范围有超过65%的重叠。在确定正常范围后，该分析仪用于确认I型糖尿病小鼠的高血糖症和诱导肾小球硬化大鼠的BUN升高。便携式分析仪对于啮齿动物的表型分析和临床护理可能是一种有价值的筛查工具，具有在实验室啮齿动物中研究自发性和实验性疾病的潜力。
