Poly Frédéric, Ewing Cheryl, Goon Scarlett, Hickey Thomas E, Rockabrand David, Majam Gary, Lee Lanfong, Phan Julie, Savarino Nicholas J, Guerry Patricia
Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
Infect Immun. 2007 Aug;75(8):3859-67. doi: 10.1128/IAI.00159-07. Epub 2007 May 21.
Cj0859c, or FspA, is a small, acidic protein of Campylobacter jejuni that is expressed by a sigma(28) promoter. Analysis of the fspA gene in 41 isolates of C. jejuni revealed two overall variants of the predicted protein, FspA1 and FspA2. Secretion of FspA occurs in broth-grown bacteria and requires a minimum flagellar structure. The addition of recombinant FspA2, but not FspA1, to INT407 cells in vitro resulted in a rapid induction of apoptosis. These data define a novel C. jejuni virulence factor, and the observed heterogeneity among fspA alleles suggests alternate virulence potential among different strains.
Cj0859c，即FspA，是空肠弯曲菌的一种小酸性蛋白，由sigma(28)启动子表达。对41株空肠弯曲菌的fspA基因分析显示，预测蛋白存在两种总体变体，即FspA1和FspA2。FspA在肉汤培养的细菌中分泌，并且需要最小的鞭毛结构。在体外将重组FspA2（而非FspA1）添加到INT407细胞中会导致细胞凋亡的快速诱导。这些数据定义了一种新的空肠弯曲菌毒力因子，并且观察到的fspA等位基因间的异质性表明不同菌株之间存在不同的毒力潜力。
