Sonestedt E, Ericson U, Gullberg B, Peñalvo J L, Adlercreutz H, Wirfält E
Department of Clinical Sciences, Lund University, Malmö, Sweden.
Eur J Clin Nutr. 2008 Aug;62(8):1005-9. doi: 10.1038/sj.ejcn.1602811. Epub 2007 May 30.
The aim of this study was to examine the variation of enterolactone from fasting and non-fasting blood of middle-aged healthy women eating a normal diet to determine the usefulness of a single sample in epidemiological studies.
Twenty-six women born between 1940 and 1950 were recruited within the Malmö Diet and Cancer cohort. Three non-fasting and two overnight fasting samples were collected from each individual during a 5-week period. Twenty-one participated in all measurements. Enterolactone concentrations were analyzed by time-resolved fluoroimmunoassay.
The within-subject and between-subject variations (coefficient of variations, CV) were estimated to 59 and 89% respectively for fasting samples and 71 and 67% for non-fasting samples. The intraclass correlation coefficients (ICC) were estimated to 0.66 (95% confidence interval (CI) 0.35-0.84) for fasting and 0.48 (95% CI, 0.22-0.72) for non-fasting samples.
Although the estimated ICC for blood samples was moderate, it indicates that enterolactone levels of both fasting and non-fasting blood samples should be useful in future projects within the Malmö Diet and Cancer cohort.
本研究旨在检测中年健康正常饮食女性空腹和非空腹血液中肠内酯的变化，以确定单一样本在流行病学研究中的实用性。
在马尔默饮食与癌症队列中招募了26名出生于1940年至1950年之间的女性。在5周内，从每位个体采集3份非空腹样本和2份过夜空腹样本。21人参与了所有测量。采用时间分辨荧光免疫分析法分析肠内酯浓度。
空腹样本的受试者内和受试者间变异（变异系数，CV）估计分别为59%和89%，非空腹样本分别为71%和67%。空腹样本的组内相关系数（ICC）估计为0.66（95%置信区间（CI）0.35 - 0.84），非空腹样本为0.48（95% CI，0.22 - 0.72）。
尽管血液样本的估计ICC为中等水平，但这表明空腹和非空腹血液样本中的肠内酯水平在马尔默饮食与癌症队列未来的项目中应是有用的。
