den Tonkelaar I, Keinan-Boker L, Veer P V, Arts C J, Adlercreutz H, Thijssen J H, Peeters P H
Julius Center for Patient-Oriented Research, University Medical Center, Utrecht, The Netherlands.
Cancer Epidemiol Biomarkers Prev. 2001 Mar;10(3):223-8.
Phytoestrogens are defined as plant substances that are structurally or functionally similar to estradiol. We report the associations of two major phytoestrogens, genistein and enterolactone, with breast cancer risk, using urinary specimens collected 1-9 years before breast cancer was diagnosed. The subjects were 88 breast cancer cases and 268 controls, selected from a cohort of postmenopausal women (n = 14,697) who participated in a breast cancer screening program. Mean levels of urinary genistein and enterolactone were determined by time resolved fluoroimmunoassay, using an average of two overnight urinary samples obtained from each participant on the first and the second screening rounds with a time interval of approximately 1 year. Odds ratios (ORs) of the highest to the lowest tertile of urinary phytoestrogen/creatinine concentrations and 95% confidence intervals (CIs) were computed. Higher urinary genistein excretion was weakly and nonsignificantly associated with a reduced breast cancer risk. OR for the highest tertile compared with lowest tertile was 0.83; 95% CI, 0.46-1.51. Higher urinary enterolactone excretion was weakly and nonsignificantly associated with an increased breast cancer risk. OR for the highest tertile compared with the lowest tertile was 1.43; 95% CI, 0.79-2.59. Tests for trends for both phytoestrogens were nonsignificant. We were not able to detect the previously reported protective effects of genistein and enterolactone on breast cancer risk in our postmenopausal population of Dutch women. Such an effect may be smaller than expected and/or limited to specific subgroups of the population.
植物雌激素被定义为在结构或功能上与雌二醇相似的植物物质。我们使用在乳腺癌确诊前1 - 9年收集的尿液样本，报告了两种主要植物雌激素（染料木黄酮和肠内酯）与乳腺癌风险的关联。研究对象为88例乳腺癌病例和268例对照，选自参与乳腺癌筛查项目的绝经后女性队列（n = 14,697）。采用时间分辨荧光免疫分析法，通过平均每位参与者在第一轮和第二轮筛查中获得的两份过夜尿液样本（时间间隔约为1年）来测定尿中染料木黄酮和肠内酯的平均水平。计算尿植物雌激素/肌酐浓度最高三分位数与最低三分位数的比值比（OR）及95%置信区间（CI）。尿中染料木黄酮排泄量较高与乳腺癌风险降低呈微弱且无显著意义的关联。最高三分位数与最低三分位数相比的OR为0.83；95% CI为0.46 - 1.51。尿中肠内酯排泄量较高与乳腺癌风险增加呈微弱且无显著意义的关联。最高三分位数与最低三分位数相比的OR为1.43；95% CI为0.79 - 2.59。两种植物雌激素的趋势检验均无显著意义。在我们荷兰绝经后女性人群中，未能检测到先前报道的染料木黄酮和肠内酯对乳腺癌风险的保护作用。这种作用可能比预期的小和/或仅限于特定亚人群体。
