Pun Sher Bahadur, Nakagomi Toyoko, Sherchand Jeevan Bahadur, Pandey Basu Dev, Cuevas Luis E, Cunliffe Nigel A, Hart C A, Nakagomi Osamu
Nagasaki University, Nagasaki, Japan.
Emerg Infect Dis. 2007 Mar;13(3):482-4. doi: 10.3201/eid1303.061367.
Of 731 stool specimens collected from children with diarrhea in Kathmandu, Nepal, from August 2004 through July 2005, 170 (23.3%) tested positive for rotavirus. Reverse transcription-PCR, including a revised G12-specific primer set, identified 56 (32.9%) as G2P[4] and 39 (23.0%) as G12 with P[6], P[8], or P[4].
2004年8月至2005年7月期间，从尼泊尔加德满都腹泻儿童中收集了731份粪便样本，其中170份（23.3%）轮状病毒检测呈阳性。逆转录聚合酶链反应（RT-PCR），包括一组经过修订的G12特异性引物，鉴定出56份（32.9%）为G2P[4]，39份（23.0%）为带有P[6]、P[8]或P[4]的G12。
