Randriamampionona Denis, Diallo Billo, Rakotoniriana Francisco, Rabemanantsoa Christian, Cheuk Kiban, Corbisier Anne-Marie, Mahillon Jacques, Ratsimamanga Suzanne, El Jaziri Mondher
Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles, Rue Adrienne Bolland 8, B-6041 Gosselies, Belgium.
Fitoterapia. 2007 Dec;78(7-8):482-9. doi: 10.1016/j.fitote.2007.03.016. Epub 2007 May 10.
A comparative quantitative analysis of the active triterpenoids in Centella asiatica samples collected in different locations in Madagascar was carried out to evaluate the natural variability in triterpenoid content and to select elite samples for further ex situ germplasm conservation and clonal propagation. The highest asiaticoside content (6.42%) was measured in samples collected in Mangoro region. In vitro propagation of C. asiatica was successfully achieved in hormone-free medium. Although lower asiaticoside content was detected in 8-week-old vitro plants, the Mangoro sample still showed the highest content in this triterpenoid constituent (1.78%). Acetoxycentellynol, a C(15)-polyacetylene, was found to be accumulated up to 18 times more in in vitro plants as compared to plant material collected in situ.
对在马达加斯加不同地点采集的积雪草样本中的活性三萜类化合物进行了比较定量分析，以评估三萜类化合物含量的自然变异性，并选择优良样本进行进一步的迁地种质保存和克隆繁殖。在芒戈罗地区采集的样本中测得的积雪草苷含量最高（6.42%）。积雪草在无激素培养基中成功实现了离体繁殖。虽然在8周龄的离体植株中检测到较低的积雪草苷含量，但芒戈罗样本在这种三萜类成分中仍显示出最高含量（1.78%）。与原位采集的植物材料相比，发现C(15)-聚乙炔乙酰氧基积雪草烯醇在离体植株中的积累量高达18倍。
