SrfI, a new type-II restriction endonuclease that recognizes the octanucleotide sequence, [sequence: see text].
作者信息
Simcox T G, Marsh S J, Gross E A, Lernhardt W, Davis S, Simcox M E
机构信息
Stratagene Cloning Systems, Inc., La Jolla, CA 92037.
出版信息
Gene. 1991 Dec 20;109(1):121-3. doi: 10.1016/0378-1119(91)90596-4.
PMID:1756971
Abstract
A new restriction endonuclease, SrfI has been isolated from an unidentified species of Streptomyces. SrfI recognizes the 8-bp palindrome, 5'-GCCCGGGC and cleaves double-stranded DNA after the third C in the sequence, producing blunt ends. SrfI is a rare-cutting enzyme and should therefore be useful for megabase mapping.
摘要
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