SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'.
作者信息
Simcox T G, Fabian L, Kretz K, Hedden V, Simcox M E
机构信息
Stratagene Cloning Systems, Inc., La Jolla, CA 92037, USA.
出版信息
Gene. 1995 Mar 21;155(1):129-30. doi: 10.1016/0378-1119(94)00711-z.
PMID:7698656
Abstract
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.
摘要
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