SanDI，一种识别5'-GG/GWCCC-3'的新型II型限制性内切酶。

SanDI, a new type-II restriction endonuclease that recognizes 5'-GG/GWCCC-3'.

作者信息

Simcox T G, Fabian L, Kretz K, Hedden V, Simcox M E

机构信息

Stratagene Cloning Systems, Inc., La Jolla, CA 92037, USA.

出版信息

Gene. 1995 Mar 21;155(1):129-30. doi: 10.1016/0378-1119(94)00711-z.

DOI:10.1016/0378-1119(94)00711-z

PMID:7698656

Abstract

A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.

摘要

一种新的限制性核酸内切酶（ENase），SanDI，已从一种未鉴定的链霉菌属物种中分离出来。SanDI识别7碱基间断回文序列5'-GG/GWCCC-3'（W = A或T），并在序列中的第二个G后切割双链DNA，产生3个核苷酸长的5'突出末端。SanDI是一种切割频率较低的核酸内切酶，因此应可用于兆碱基图谱绘制和载体构建。