Willner Itamar, Zayats Maya
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Angew Chem Int Ed Engl. 2007;46(34):6408-18. doi: 10.1002/anie.200604524.
The selection of aptamers-nucleic acids that specifically bind low-molecular-weight substrates or proteins-by the SELEX (systematic evolution of ligands by exponential enrichment) procedure has attracted recent efforts directed to the development of new specific recognition units. In particular, extensive activities have been directed to the application of aptamers as versatile materials for the design of biosensors. The Minireview summarizes the recent accomplishments in developing electronic aptamer-based sensors (aptasensors), which include electrochemical, field-effect transistor, and microgravimetric quartz crystal microbalance sensors, and describes methods to develop amplified aptasensor devices and label-free aptasensors.
通过指数富集配体系统进化（SELEX）程序筛选适配体（即能特异性结合低分子量底物或蛋白质的核酸），这一方法近来已吸引了诸多致力于开发新型特异性识别元件的研究工作。特别是，大量研究活动聚焦于将适配体用作生物传感器设计的通用材料。本综述总结了基于电子适配体的传感器（适配体传感器）开发方面的最新成果，其中包括电化学传感器、场效应晶体管传感器和微重力石英晶体微天平传感器，并描述了开发放大型适配体传感器装置和无标记适配体传感器的方法。
