Iwatsuki Masato, Uchida Ryuji, Takakusagi Yoichi, Matsumoto Atsuko, Jiang Cheng-Lin, Takahashi Yoko, Arai Masayoshi, Kobayashi Susumu, Matsumoto Makoto, Inokoshi Junji, Tomoda Hiroshi, Omura Satoshi
The Kitasato Institute, Shirokane, Tokyo, Japan.
J Antibiot (Tokyo). 2007 Jun;60(6):357-63. doi: 10.1038/ja.2007.48.
Two anti-mycobacterial peptides with a lasso structure, named lariatins A and B, were separated by HP-20 and ODS column chromatographies and purified by HPLC from the culture broth of Rhodococcus jostii K01-B0171, which was isolated from soil aggregates collected in Yunnan, China. Lariains A and B showed growth inhibition against Mycobacterium smegmatis with MIC values of 3.13 and 6.25 microg/ml in agar dilution method, respectively. Furthermore, lariatin A inhibited the growth of Mycobacterium tuberculosis with an MIC of 0.39 microg/ml in liquid microdilution method.
从中国云南采集的土壤团聚体中分离得到的约氏红球菌K01-B0171的培养液中，通过HP-20和ODS柱色谱法分离出两种具有套索结构的抗分枝杆菌肽，命名为套索菌素A和B，并通过HPLC进行纯化。在琼脂稀释法中，套索菌素A和B对耻垢分枝杆菌均表现出生长抑制作用，MIC值分别为3.13和6.25微克/毫升。此外，在液体微量稀释法中，套索菌素A对结核分枝杆菌的生长具有抑制作用，MIC为0.39微克/毫升。
