Oliveira Guilherme
Laboratory of Cellular and Molecular Parasitology, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte, MG 30190-002, Brazil.
Exp Parasitol. 2007 Nov;117(3):229-35. doi: 10.1016/j.exppara.2007.06.001. Epub 2007 Jun 12.
Large scale EST sequencing projects have been carried out for Schistosoma mansoni and Schistosoma japonicum. This update will briefly review the most recent accomplishments in the area and discuss the use of EST data for the purposes of gene discovery, gene model development, genome annotation and SNP analysis. In addition, the use of ESTs for studying other features of the transcriptome such as splice site and transcription initiation variants will be discussed as well as approaches to assigning function to unknown transcripts. Although EST sequencing has contributed much for schistosome research, other data mining possibilities exist, including the identification of putative drug and vaccine targets.
已经针对曼氏血吸虫和日本血吸虫开展了大规模的EST测序项目。本综述将简要回顾该领域的最新成果，并讨论EST数据在基因发现、基因模型开发、基因组注释和SNP分析中的应用。此外，还将讨论利用EST研究转录组的其他特征，如剪接位点和转录起始变体，以及为未知转录本赋予功能的方法。尽管EST测序为血吸虫研究做出了很大贡献，但还存在其他数据挖掘的可能性，包括鉴定潜在的药物和疫苗靶点。
