Brown Robert A, Phillips James B
Tissue Regeneration & Engineering Center, Institute of Orthopedics, University College London, Stanmore Campus, London, HA7 4LP, United Kingdom.
Int Rev Cytol. 2007;262:75-150. doi: 10.1016/S0074-7696(07)62002-6.
Basic science research in tissue engineering and regenerative medicine aims to investigate and understand the deposition, growth, and remodeling of tissues by drawing together approaches from a range of disciplines. This review discusses approaches that use biomimetic proteins and cellular therapies, both in the development of clinical products and of model platforms for scientific investigation. Current clinical approaches to repairing skin, bone, nerve, heart valves, blood vessels, ligaments, and tendons are described and their limitations identified. Opportunities and key questions for achieving clinical goals are discussed through commonly used examples of biomimetic scaffolds: collagen, fibrin, fibronectin, and silk. The key questions addressed by three-dimensional culture models, biomimetic materials, surface chemistry, topography, and their interaction with cells in terms of durotaxis, mechano-regulation, and complex spatial cueing are reviewed to give context to future strategies for biomimetic technology.
组织工程与再生医学的基础科学研究旨在通过整合一系列学科的方法来研究和理解组织的沉积、生长和重塑。本综述讨论了在临床产品开发和科学研究模型平台中使用仿生蛋白和细胞疗法的方法。描述了目前修复皮肤、骨骼、神经、心脏瓣膜、血管、韧带和肌腱的临床方法，并指出了它们的局限性。通过常用的仿生支架实例：胶原蛋白、纤维蛋白、纤连蛋白和丝绸，讨论了实现临床目标的机会和关键问题。综述了三维培养模型、仿生材料、表面化学、拓扑结构及其在趋硬性、机械调节和复杂空间线索方面与细胞相互作用所涉及的关键问题，为仿生技术的未来策略提供背景。
