Weber J M, Greenlees J L, Stewart R B
Infect Immun. 1976 Feb;13(2):326-31. doi: 10.1128/iai.13.2.326-331.1976.
The production of interferon from Newcastle disease virus-infected mouse L929 cells was investigated in relation to superinduction procedures, cell density, cellular cyclic adenosine 3',5'-monophosphate (cAMP) levels, and rate of incorporation of 14C-labeled protein hydrolysate into trichloroacetic acid-precipitable material. Densely populated cultures did not have their interferon production enhanced through "superinduction" using cycloheximide, actinomycin D, or the two antimetabolites in combination. These dense cultures produced more interferon per cell than less dense cultures, even though the interferon production from the latter cells could be enhanced two- to threefold by cycloheximide or combined cycloheximide and actinomycin D. Cells in densely populated cultures relative to those from sparsely populated cultures were smaller in volume, had a correspondingly reduced protein content and a lower concentration of cAMP, and were less able to concentrate 14C-labeled protein hydrolysate, although proportionally they were just as efficient in incorporating labeled precursors into trichloroacetic acid-precipitable polypeptides.
研究了新城疫病毒感染的小鼠L929细胞产生干扰素的情况，涉及超诱导程序、细胞密度、细胞内环磷酸腺苷（cAMP）水平以及14C标记的蛋白水解产物掺入三氯乙酸沉淀物质的速率。高密度培养的细胞，通过使用环己酰亚胺、放线菌素D或这两种抗代谢物联合进行“超诱导”，其干扰素产量并未增加。这些高密度培养的细胞比低密度培养的细胞每个细胞产生更多的干扰素，尽管后者细胞的干扰素产量可通过环己酰亚胺或环己酰亚胺与放线菌素D联合使用而提高两到三倍。与稀疏培养的细胞相比，高密度培养的细胞体积较小，蛋白质含量相应降低，cAMP浓度较低，并且浓缩14C标记的蛋白水解产物的能力较弱，尽管按比例它们将标记前体掺入三氯乙酸沉淀多肽的效率相同。
