Science. 1982 Jan 15;215(4530):291-3. doi: 10.1126/science.215.4530.291.
Steady-state turnover times for simultaneous photosynthetic production of hydrogen and oxygen have been measured for two systems: the in vitro system comprised of isolated chloroplasts, ferredoxin, and hydrogenase, and the anaerobically adapted green alga Chlamydomonas reinhardtii [137c(+) mating type]. In both systems, the simultaneous photoproduction of hydrogen and oxygen was measured by driving the systems into the steady state with repetitive, single-turnover, flash illumination. The turnover times for production of both oxygen and hydrogen in photosynthetic water splitting are in milliseconds and are equal to or less than the turnover time for carbon dioxide reduction in intact algal cells. The oxygen and hydrogen turnover times are therefore compatible with each other and partially compatible with the excitation rate of the photosynthetic reaction centers under conditions of solar irradiation.
体外系统由分离的叶绿体、铁氧还蛋白和氢化酶组成，以及厌氧适应的绿藻莱茵衣藻[137c(+)交配型]。在这两个系统中，通过用重复的单周转闪光照射将系统驱动到稳定状态，来测量氢气和氧气的同时光生产。光合水分解中产生氧气和氢气的周转率均在毫秒范围内，与完整藻类细胞中二氧化碳还原的周转率相等或更短。因此，氧气和氢气的周转率彼此兼容，并且在太阳辐照条件下与光合反应中心的激发率部分兼容。
