Allison Bradley C, Applegate Bruce M, Youngblood Jeffrey P
Department of Materials Engineering and Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Biomacromolecules. 2007 Oct;8(10):2995-9. doi: 10.1021/bm7004627. Epub 2007 Sep 18.
Quaternized poly(vinylpyridine) (PVP) is a polymer with inherent antimicrobial properties that is effective against Gram-positive bacteria, Gram-negative bacteria, viruses, and yeast cells. However, quaternized PVP has poor biocompatibility, which prevents its use in biomaterial applications. Copolymerization was examined as a method of modifying the structure to incorporate biocompatibility. Polyethyleneglycol methyl ether methacrylate (PEGMA) and hydroxyethyl methacrylate (HEMA) are polymers generally known to be biocompatible and thus were chosen as comonomers. Random copolymers of 4-vinylpyridine and PEGMA or HEMA were synthesized via free radical polymerization and quaternized with bromohexane. Copolymer biocompatibility was characterized by interaction with human red blood cells to analyze hemolysis. Hemolysis of human red blood cells was conducted on insoluble films and on water-soluble polymers in a serial dilution study. Hemolysis results demonstrated that blood compatibility does not depend on PEG chain length in PEGMA incorporated copolymers. Results indicate a critical weight ratio of PEGMA to VP in copolymers separating the no-hemolysis regime from 100% hemolysis.
季铵化聚（乙烯基吡啶）（PVP）是一种具有固有抗菌特性的聚合物，对革兰氏阳性菌、革兰氏阴性菌、病毒和酵母细胞均有效。然而，季铵化PVP的生物相容性较差，这使其无法用于生物材料应用。研究了共聚作为一种修饰结构以引入生物相容性的方法。聚乙二醇甲基醚甲基丙烯酸酯（PEGMA）和甲基丙烯酸羟乙酯（HEMA）是通常已知具有生物相容性的聚合物，因此被选作共聚单体。通过自由基聚合合成了4-乙烯基吡啶与PEGMA或HEMA的无规共聚物，并用溴己烷进行季铵化。通过与人类红细胞相互作用来分析溶血情况，以此表征共聚物的生物相容性。在连续稀释研究中，对不溶性薄膜和水溶性聚合物进行了人类红细胞溶血实验。溶血结果表明，在含PEGMA的共聚物中，血液相容性并不取决于PEG链的长度。结果表明，共聚物中PEGMA与VP的临界重量比将无溶血状态与100%溶血状态区分开来。
