直接检测装置在透射电子显微镜中的应用。
Applications of direct detection device in transmission electron microscopy.
作者信息
Jin Liang, Milazzo Anna-Clare, Kleinfelder Stuart, Li Shengdong, Leblanc Philippe, Duttweiler Fred, Bouwer James C, Peltier Steven T, Ellisman Mark H, Xuong Nguyen-Huu
机构信息
University of California at San Diego, La Jolla, CA 92093, USA.
出版信息
J Struct Biol. 2008 Mar;161(3):352-8. doi: 10.1016/j.jsb.2007.10.007. Epub 2007 Oct 26.
A prototype direct detection device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120-400 keV beam energies (Xuong et al., 2007. Methods in Cell Biology, 79, 721-739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5 microm). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system.
一种原型直接检测设备（DDD）相机系统在提高120 - 400 keV束流能量下电子显微镜的空间分辨率和信噪比方面显示出了巨大的潜力（Xuong等人，2007年。《细胞生物学方法》，79卷，721 - 739页）。与电荷耦合器件（CCD）不同，DDD相机无需使用限制分辨率的闪烁屏，由于其像素尺寸小（5微米），在空间分辨率方面能够超越基于CCD的系统。在本文中，对DDD原型的调制传递函数（MTF）进行了测量，并与商用科学CCD相机系统的规格进行了比较。将DDD的快速速度与图像拼接技术相结合，现在可以实现快速大面积成像。本文展示了来自DDD相机的首张大面积拼接图像和首个断层扫描数据集，以及一种利用DDD系统的快速读出功能来校正样本漂移的图像处理算法。
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