Abdukalykova S T, Zhao X, Ruiz-Feria C A
Department of Animal Science, McGill University, Ste Anne de Bellevue, Québec, Canada.
Poult Sci. 2008 Jan;87(1):50-5. doi: 10.3382/ps.2007-00315.
We examined the effects of vitamin E (VE) and Arg on the subpopulations of T lymphocytes in peripheral blood after an infectious bursal disease virus vaccination (20 d old, n = 54). Broiler chickens were fed diets with normal levels of Arg (NARG, 1.2%) or high levels of Arg (HARG, 2.2%) and 3 levels of VE (40, 80, or 200 IU/kg of feed) in a factorial arrangement of treatments. The percentages of CD4+ and CD8+ cells 9 d after vaccination were not different in birds fed the HARG or NARG feed, but they were higher in birds fed the VE80 diet than in birds fed the VE40 diet. Birds fed the VE200 feed had similar levels of CD4+ and CD8+ cells as birds fed the VE40 diet. However, 19 d after vaccination, the percentage of CD4+ cells was higher in birds fed the HARG (43.93 +/- 1.05) diet than in birds fed the NARG diet (41.14 +/- 1.05) and in birds fed the VE80 and VE200 (44.1 +/- 1.28 and 44.42 +/- 1.28, respectively) diets compared with birds fed the VE40 diet (39.04 +/- 1.28). On the other hand, the percentage of CD8+ cells was highest in birds fed the HARG-VE80 feed compared with all the other Arg and VE combinations. The percentages of CD3+ cells and BU-1+ cells were higher in birds fed the HARG (81.35 +/- 1.46 and 22.3 +/- 0.89, respectively) diet than in birds fed the NARG diets (77.82 +/- 1.46 and 18.93 +/- 0.89, respectively). Birds fed the VE40 had a higher amount of BU-1+ (24.35 +/- 1.073) but lower amounts of CD3+ cells (75.76 +/- 1.76) than birds fed the VE80 (18.46 +/- 1.073 and 81.67 +/- 1.76) and VE200 (19.03 +/- 1.073 and 81.19 +/- 1.76, respectively). Neither Arg nor VE had an effect on the CD4+:CD8+ cell ratio and on the percentage of immature (CD4+CD8+) T lymphocytes 9 d after vaccination. These results suggest that Arg and VE have complimentary effects on cellular and humoral immune function and may enhance the resistance of broilers to infectious diseases.
我们研究了维生素E（VE）和精氨酸（Arg）对传染性法氏囊病病毒疫苗接种后（20日龄，n = 54）外周血T淋巴细胞亚群的影响。将肉鸡按照析因设计的处理方式，饲喂含正常水平精氨酸（NARG，1.2%）或高水平精氨酸（HARG，2.2%）以及3种水平VE（40、80或200 IU/kg饲料）的日粮。接种疫苗9天后，饲喂HARG或NARG饲料的鸡中CD4⁺和CD8⁺细胞的百分比没有差异，但饲喂VE80日粮的鸡中的这些细胞百分比高于饲喂VE40日粮的鸡。饲喂VE200饲料的鸡的CD4⁺和CD8⁺细胞水平与饲喂VE40日粮的鸡相似。然而，接种疫苗19天后，饲喂HARG（43.93±1.05）日粮的鸡中CD4⁺细胞的百分比高于饲喂NARG日粮（41.14±1.05）的鸡，并且与饲喂VE40日粮（39.04±1.28）的鸡相比，饲喂VE80和VE200日粮（分别为44.1±1.28和44.42±1.28）的鸡中的CD4⁺细胞百分比更高。另一方面，与所有其他精氨酸和VE组合相比，饲喂HARG-VE80饲料的鸡中CD8⁺细胞的百分比最高。饲喂HARG日粮（分别为81.35±1.46和22.3±0.89）的鸡中CD3⁺细胞和BU-1⁺细胞的百分比高于饲喂NARG日粮（分别为77.82±1.46和18.93±0.89）的鸡。与饲喂VE80（分别为18.46±1.073和81.67±1.76）和VE200（分别为19.03±1.073和81.19±1.76）的鸡相比，饲喂VE40的鸡的BU-1⁺细胞数量较多（24.35±1.073），但CD3⁺细胞数量较少（75.76±1.76）。接种疫苗9天后，精氨酸和VE对CD4⁺:CD8⁺细胞比例以及未成熟（CD4⁺CD8⁺）T淋巴细胞百分比均无影响。这些结果表明，精氨酸和VE对细胞免疫和体液免疫功能具有互补作用，可能增强肉鸡对传染病的抵抗力。
