Suppr超能文献

2006年比利时疫情期间抗体酶联免疫吸附测定法(ELISA)和实时逆转录聚合酶链反应(RT-PCR)用于蓝舌病病毒8型诊断及特征分析的评估

Evaluation of antibody-ELISA and real-time RT-PCR for the diagnosis and profiling of bluetongue virus serotype 8 during the epidemic in Belgium in 2006.

作者信息

Vandenbussche Frank, Vanbinst Tine, Verheyden Bart, Van Dessel Wesley, Demeestere Lien, Houdart Philippe, Bertels Guido, Praet Nicolas, Berkvens Dirk, Mintiens Koen, Goris Nesya, De Clercq Kris

机构信息

Veterinary and Agrochemical Research Centre, Department of Virology, Groeselenberg 99, B-1180 Brussels, Belgium.

出版信息

Vet Microbiol. 2008 May 25;129(1-2):15-27. doi: 10.1016/j.vetmic.2007.10.029. Epub 2007 Nov 7.

Abstract

In 2006 bluetongue (BT) emerged for the first time in North-Western Europe. Reliable diagnostic tools are essential in controlling BT but data on the diagnostic sensitivity (Se) and specificity (Sp) are often missing. This paper aims to describe and analyse the results obtained with the diagnostics used in Belgium during the 2006 BT crisis. The diagnosis was based on a combination of antibody detection (competitive ELISA, cELISA) and viral RNA detection by real-time RT-PCR (RT-qPCR). The performance of the cELISA as a diagnostic tool was assessed on field results obtained during the epidemic and previous surveillance campaigns. As the infectious status of the animals is unknown during an epidemic, a Bayesian analysis was performed. Both assays were found to be equally specific (RT-qPCR: 98.5%; cELISA: 98.2%) while the diagnostic sensitivity of the RT-qPCR (99.5%) was superior to that of the cELISA (87.8%). The assumption of RT-qPCR as standard of comparison during the bluetongue virus (BTV) epidemic proved valid based on the results of the Bayesian analysis. A ROC analysis of the cELISA, using RT-qPCR as standard of comparison, showed that the cut-off point with the highest accuracy occurred at a percentage negativity of 66, which is markedly higher than the cut-off proposed by the manufacturer. The analysis of the results was further extended to serological and molecular profiling and the possible use of profiling as a rapid epidemiological marker of the BTV in-field situation was assessed. A comparison of the serological profiles obtained before, during and at the end of the Belgian epidemic clearly showed the existence of an intermediate zone which appears soon after BTV (re)enters the population. The appearance or disappearance of this intermediate zone is correlated with virus circulation and provides valuable information, which would be entirely overlooked if only positive and negative results were considered.

摘要

2006年,蓝舌病(BT)首次在欧洲西北部出现。可靠的诊断工具对于控制蓝舌病至关重要,但关于诊断敏感性(Se)和特异性(Sp)的数据往往缺失。本文旨在描述和分析2006年比利时蓝舌病危机期间所使用诊断方法的结果。诊断基于抗体检测(竞争ELISA,cELISA)和实时逆转录聚合酶链反应(RT-qPCR)检测病毒RNA相结合。根据疫情期间及之前监测活动获得的现场结果评估了cELISA作为诊断工具的性能。由于疫情期间动物的感染状况未知,因此进行了贝叶斯分析。发现两种检测方法的特异性相当(RT-qPCR:98.5%;cELISA:98.2%),而RT-qPCR的诊断敏感性(99.5%)优于cELISA(87.8%)。基于贝叶斯分析结果,在蓝舌病病毒(BTV)疫情期间将RT-qPCR作为比较标准的假设被证明是有效的。以RT-qPCR作为比较标准对cELISA进行的ROC分析表明,准确率最高的截断点出现在阴性百分比为66时,这明显高于制造商建议的截断点。结果分析进一步扩展到血清学和分子谱分析,并评估了谱分析作为BTV现场情况快速流行病学标志物的可能用途。对比比利时疫情之前、期间和结束时获得的血清学谱,清楚地显示存在一个中间区,该中间区在BTV(重新)进入种群后不久出现。这个中间区的出现或消失与病毒传播相关,并提供了有价值的信息,如果只考虑阳性和阴性结果,这些信息将被完全忽略。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验