Liu T, She R, Wang K, Bao H, Zhang Y, Luo D, Hu Y, Ding Y, Wang D, Peng K
College Of Veterinary Medicine, China Agricultural University, Beijing, 100094 China.
Poult Sci. 2008 Feb;87(2):250-4. doi: 10.3382/ps.2007-00353.
Ninety chickens were randomly divided into 2 groups (45 chickens in each group) to determine the effect of oral administration of rabbit sacculus rotundus antimicrobial peptides (RSRP) on the intestinal mucosal immune responses in chicken. On d 7, 14, 21, and 28, the animals received 0.1 mg of RSRP dissolved in 0.5 mL of physiological saline. The control groups received the same dose of physiological salt solution on the same day. The results showed that RSRP increased the villus height of the duodenum (P < 0.01) and jejunum (P < 0.05) at the ages of 28, 42, and 56 d. The numbers of intestinal intraepithelial lymphocytes in different parts of intestine of the RSRP group were increased significantly more than that of the control (P < 0.01 or P < 0.05) at the ages of 28, 42, and 56 d. The RSRP increased the area of IgA-secreting cells of each fragment of intestine at all 3 time points. These results indicated that the presence of RSRP affected and considerably modified the structure of the intestine and mucosal immune parameters in healthy chickens when compared with controls.
将90只鸡随机分为2组（每组45只），以确定口服兔圆小囊抗菌肽（RSRP）对鸡肠道黏膜免疫反应的影响。在第7、14、21和28天，给动物口服溶解于0.5 mL生理盐水中的0.1 mg RSRP。对照组在同一天接受相同剂量的生理盐溶液。结果表明，在28、42和56日龄时，RSRP增加了十二指肠（P < 0.01）和空肠（P < 0.05）的绒毛高度。在28、42和56日龄时，RSRP组肠道不同部位的肠上皮内淋巴细胞数量显著高于对照组（P < 0.01或P < 0.05）。在所有3个时间点，RSRP均增加了各肠段IgA分泌细胞的面积。这些结果表明，与对照组相比，RSRP的存在影响并显著改变了健康鸡的肠道结构和黏膜免疫参数。
