Wang D, Ma W, She R, Sun Qu, Liu Y, Hu Y, Liu L, Yang Y, Peng K
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Poult Sci. 2009 May;88(5):967-74. doi: 10.3382/ps.2008-00533.
Sixty specific-pathogen-free chickens were randomly divided into 2 groups (30 chickens for each group) to determine the effect of swine gut antimicrobial peptides (SGAMP) on intestinal mucosal immunity. All chickens were raised in negative-pressure isolators and fed the same diet. The results were as follows. (1) In the SGAMP group, the number of mast cells was increased markedly in the duodenum from d 21 to 49 (P < 0.05), and similar results were observed in the jejunum and ileum after being treated with SGAMP (P < 0.05). However, fewer mast cells were observed in those same tissues in the control. (2) Intraepithelial lymphocytes in the duodenum, jejunum, and ileum were increased significantly from d 21 to 49 in the SGAMP group compared with the control (P < 0.05). (3) Compared with the control, goblet cells were increased significantly in duodenum and jejunum from d 35 to 49 (P < 0.05) after SGAMP treatment and in the ileum were increased from d 21 to 49 (P < 0.05). (4) Swine gut antimicrobial peptides upgrade the expression of secretory IgA at different sites within the intestinal tract. The results strongly support that SGAMP can enhance the intestinal mucosal immune parameters of specific-pathogen-free chickens. Our research contributes to the further understanding of immunoregulatory mechanisms of intestinal mucosal immunity and the contribution of SGAMP to this process.
将60只无特定病原体鸡随机分为2组（每组30只），以确定猪肠道抗菌肽（SGAMP）对肠道黏膜免疫的影响。所有鸡均饲养在负压隔离器中，并给予相同的饲料。结果如下：（1）在SGAMP组中，从第21天到49天，十二指肠中肥大细胞数量显著增加（P<0.05），用SGAMP处理空肠和回肠后也观察到类似结果（P<0.05）。然而，对照组相同组织中的肥大细胞较少。（2）与对照组相比，SGAMP组十二指肠、空肠和回肠中的上皮内淋巴细胞从第21天到49天显著增加（P<0.05）。（3）与对照组相比，SGAMP处理后，十二指肠和空肠中杯状细胞从第35天到49天显著增加（P<0.05），回肠中杯状细胞从第21天到49天增加（P<0.05）。（4）猪肠道抗菌肽可提升肠道不同部位分泌型IgA的表达。结果有力地支持了SGAMP可增强无特定病原体鸡的肠道黏膜免疫参数。我们的研究有助于进一步了解肠道黏膜免疫的免疫调节机制以及SGAMP在此过程中的作用。
