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绵羊布鲁氏菌和海兽布鲁氏菌中IS711转座的证明。

Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis.

作者信息

Ocampo-Sosa Alain A, García-Lobo Juan M

机构信息

Departamento de Biología Molecular, Universidad de Cantabria, Instituto de Biomedicina y Biotecnología de Cantabria, IBBTEC, CSIC-Universidad de Cantabria-IDICAN, Santander, Spain.

出版信息

BMC Microbiol. 2008 Jan 24;8:17. doi: 10.1186/1471-2180-8-17.

DOI:10.1186/1471-2180-8-17
PMID:18218072
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2266754/
Abstract

BACKGROUND

The Brucella genome contains an insertion sequence (IS) element called IS711 or IS6501, which is specific to the genus. The copy number of IS711 varies in the genome of the different Brucella species, ranging from 7 in B. abortus, B. melitensis and B. suis to more than 30 in B. ovis and in Brucella strains isolated from marine mammals. At present, there is no experimental evidence of transposition of IS711, but the occurrence of this element with a high copy number in some species, and the isolation of Brucella strains with "ectopic" copies of IS711 suggested that this IS could still transpose.

RESULTS

In this study we obtained evidence of transposition of IS711 from the B. ovis and B. pinnipedialis chromosomes by using the "transposon trap" plasmid pGBG1. This plasmid expresses resistance to tetracycline only if the repressor gene that it contains is inactivated. The strains B. melitensis 16 M, B. abortus RB51, B. ovis BOC22 (field strain) and B. pinnipedialis B2/94, all containing the plasmid pGBG1, were grown in culture media with tetracycline until the appearance of tetracycline resistant mutants (TcR). TcR mutants due to IS711 transposition were only detected in B. ovis and B. pinnipedialis strains.

CONCLUSION

Four different copies of IS711 were found to transpose to the same target sequence in the plasmid pGBG1. This demonstrated that IS711 are active in vivo, specially in Brucella species with a high number of IS711 copies as B. ovis and B. pinnipedialis.

摘要

背景

布鲁氏菌基因组包含一种名为IS711或IS6501的插入序列（IS）元件，该元件是该属特有的。IS711的拷贝数在不同布鲁氏菌物种的基因组中有所不同，从流产布鲁氏菌、马尔他布鲁氏菌和猪布鲁氏菌中的7个到绵羊布鲁氏菌和从海洋哺乳动物分离出的布鲁氏菌菌株中的30多个不等。目前，尚无IS711转座的实验证据，但该元件在某些物种中以高拷贝数出现，以及分离出具有IS711“异位”拷贝的布鲁氏菌菌株，表明该IS仍可能发生转座。

结果

在本研究中，我们通过使用“转座子捕获”质粒pGBG1获得了IS711从绵羊布鲁氏菌和海狗布鲁氏菌染色体中转座的证据。该质粒仅在其包含的阻遏基因失活时才表达对四环素的抗性。含有质粒pGBG1的马尔他布鲁氏菌16M、流产布鲁氏菌RB51、绵羊布鲁氏菌BOC22（田间菌株）和海狗布鲁氏菌B2/94在含有四环素的培养基中培养，直到出现四环素抗性突变体（TcR）。仅在绵羊布鲁氏菌和海狗布鲁氏菌菌株中检测到由于IS711转座导致的TcR突变体。

结论

发现四个不同的IS711拷贝转座到质粒pGBG1中的相同靶序列。这证明IS711在体内具有活性，特别是在具有大量IS711拷贝的布鲁氏菌物种中，如绵羊布鲁氏菌和海狗布鲁氏菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/bee5272de197/1471-2180-8-17-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/25a2a07b2ec0/1471-2180-8-17-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/723c8b423755/1471-2180-8-17-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/d5277f520bea/1471-2180-8-17-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/5391ab1b2d84/1471-2180-8-17-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/bee5272de197/1471-2180-8-17-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/25a2a07b2ec0/1471-2180-8-17-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/723c8b423755/1471-2180-8-17-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/d5277f520bea/1471-2180-8-17-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/5391ab1b2d84/1471-2180-8-17-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/739a/2266754/bee5272de197/1471-2180-8-17-5.jpg

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