Smith H G, Horowitz N, Silverman N A, Henson D E, Chretien P B
Cancer. 1976 Sep;38(3):1155-62. doi: 10.1002/1097-0142(197609)38:3<1155::aid-cncr2820380317>3.0.co;2-4.
Antibodies to herpes simplex viral-induced antigens (HSVIA) were assayed by an indirect immunofluorescent technique in 93 regular cigarette smokers, 75 of whom also imbibed alcoholic beverages. Controls were 94 nonsmoking, nondrinking volunteers matched with the smoker for age and sex. IgA antibodies to HSVIA were detected six times more frequently in the sera of smokers than in nonsmoking controls, p less than .0005. IgG and IgM anti-HSVIA were detected with comparable frequencies in both groups, but the antibody titers were significantly higher in the smoking group than in controls, p less than .05. IgA anti-HSVIA was detected more frequently in smokers with 10 or more pack-years of cigarette exposure than in smokers with 10 or more pack-years of smoking (p less than .05) or in matched nonsmokers (p less than .02). IgA antibody titers to HSVIA were significantly higher in cigarette smokers who drank alcoholic beverages than in smokers who did not drink, p less than .025. This study demonstrates that cigarette smoking and the use of alcoholic beverages are associated with heightened humoral immunity to HSV-induced antigens in a population at high risk for development of squamous cell carcinomas of the head and neck region.
采用间接免疫荧光技术对93名经常吸烟的人进行了单纯疱疹病毒诱导抗原（HSVIA）抗体检测，其中75人还饮用酒精饮料。对照组为94名不吸烟、不饮酒的志愿者，年龄和性别与吸烟者匹配。吸烟者血清中HSVIA的IgA抗体检出频率比不吸烟对照组高6倍，p值小于0.0005。两组中IgG和IgM抗HSVIA的检出频率相当，但吸烟组的抗体滴度显著高于对照组，p值小于0.05。每天吸烟10包年及以上的吸烟者中HSVIA的IgA抗体检出频率高于每天吸烟不足10包年的吸烟者（p值小于0.05）或匹配的不吸烟者（p值小于0.02）。饮用酒精饮料的吸烟者中HSVIA的IgA抗体滴度显著高于不饮酒的吸烟者，p值小于0.025。这项研究表明，在头颈部鳞状细胞癌高发人群中，吸烟和饮用酒精饮料与对HSV诱导抗原的体液免疫增强有关。
