Cattoir Vincent, Poirel Laurent, Aubert Camille, Soussy Claude-James, Nordmann Patrice
Institut Nationale de la Santé et de la Recherche Médicale Unité 914, Le Kremlin-Bicêtre, France.
Emerg Infect Dis. 2008 Feb;14(2):231-7. doi: 10.3201/eid1402.070677.
We searched for plasmid-mediated quinolone resistance determinants of the Qnr type in several water samples collected at diverse locations from the Seine River (Paris, France). The qnrS2 genes were identified from Aeromonas punctata subsp. punctata and A. media. The qnrS2 gene was located on IncU-type plasmids in both isolates, which resulted in increased MIC values of quinolones and fluoroquinolones, once they were transferred into Escherichia coli. The qnrS2 gene identified in A. punctata was part of novel genetic structure corresponding to a mobile insertion cassette element. This identification of plasmid-mediated qnr genes outside Enterobacteriaceae underlines a possible diffusion of those resistance determinants within gram-negative rods.
我们在从塞纳河（法国巴黎）不同地点采集的多个水样中，寻找Qnr型质粒介导的喹诺酮耐药决定子。从点状气单胞菌点状亚种和中间气单胞菌中鉴定出了qnrS2基因。在这两个分离株中，qnrS2基因均位于IncU型质粒上，一旦将其转入大肠杆菌，会导致喹诺酮类和氟喹诺酮类药物的最低抑菌浓度（MIC）值升高。在点状气单胞菌中鉴定出的qnrS2基因是与一个移动插入盒元件相对应的新型遗传结构的一部分。在肠杆菌科以外鉴定出质粒介导的qnr基因，凸显了这些耐药决定子在革兰氏阴性杆菌中可能的扩散情况。
