Hipp Jennifer, Atala Anthony
Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC, 27157, USA.
Stem Cell Rev. 2008 Spring;4(1):3-11. doi: 10.1007/s12015-008-9010-8.
The shortage of organ donors for regenerative medicine has stimulated research on stem cells as a potential resource for cell-based therapy. Stem cells have been used widely for regenerative medicine applications. The development of innovative methods to generate stem cells from different sources suggests that there may be new alternatives for cell-based therapies. Here, we provide an overview of human embryonic stem cells (hES) and the methods for obtaining these cells and other broadly multipotent or pluripotent cell types. These methods include somatic cell nuclear transfer, single cell embryo biopsy, arrested embryos, altered nuclear transfer, and reprogramming somatic cells. We also discuss the use of amniotic-fluid derived stem cells (AFS) for potential patient-specific therapies.
再生医学中器官供体的短缺推动了对干细胞的研究，干细胞有望成为细胞疗法的潜在资源。干细胞已广泛应用于再生医学领域。从不同来源生成干细胞的创新方法不断涌现，这表明基于细胞的疗法可能会有新的替代方案。在此，我们概述了人类胚胎干细胞（hES）以及获取这些细胞和其他广泛多能或全能细胞类型的方法。这些方法包括体细胞核移植、单细胞胚胎活检、停滞胚胎、改良核移植和体细胞重编程。我们还讨论了羊水来源干细胞（AFS）在潜在的针对特定患者疗法中的应用。
