Zarantonelli M L, Lancellotti M, Deghmane A E, Giorgini D, Hong E, Ruckly C, Alonso J-M, Taha M-K
Neisseria Unit, National Reference Centre for Meningococci, Institut Pasteur, Paris, France.
Clin Microbiol Infect. 2008 May;14(5):467-72. doi: 10.1111/j.1469-0691.2008.01955.x. Epub 2008 Feb 22.
Clinical isolates of Neisseria meningitidis from cases of meningococcal disease, collected between January 2000 and December 2004, were identified and typed at the French National Reference Centre. A representative subset of 546 isolates from among 2882 isolates was further genotyped by multilocus sequence typing to determine their genetic lineages (clonal complexes) and the degree of diversification among different clonal complexes. Representative isolates of the main clonal complexes were tested for their virulence in mice and for proapoptotic effects on human epithelial cells. High genetic diversity in some genetic lineages (ST-32 and ST-41/44) was correlated with heterogeneity in virulence in mice and proapoptotic effects on human epithelial cells. In contrast, the homogeneous genetic structure of isolates of the ST-11 clonal complex, regardless of their serogroup, correlated positively with a fatal outcome of the infection, increased virulence in mice and increased proapoptotic effects on human epithelial cells.
2000年1月至2004年12月期间收集的脑膜炎球菌病病例中的脑膜炎奈瑟菌临床分离株，在法国国家参考中心进行了鉴定和分型。从2882株分离株中选取了546株具有代表性的子集，通过多位点序列分型进一步进行基因分型，以确定它们的遗传谱系（克隆复合体）以及不同克隆复合体之间的多样化程度。对主要克隆复合体的代表性分离株进行了小鼠毒力测试以及对人上皮细胞的促凋亡作用测试。某些遗传谱系（ST-32和ST-41/44）中的高遗传多样性与小鼠毒力的异质性以及对人上皮细胞的促凋亡作用相关。相比之下，无论血清群如何，ST-11克隆复合体分离株的同质遗传结构与感染的致命结局、小鼠毒力增加以及对人上皮细胞的促凋亡作用增加呈正相关。
