Romanenko Lyudmila A, Uchino Masataka, Tebo Bradley M, Tanaka Naoto, Frolova Galina M, Mikhailov Valery V
Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, Vladivostok 690022, Russia.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):706-10. doi: 10.1099/ijs.0.65406-0.
An aerobic, Gram-negative, motile, non-pigmented bacterium, strain KMM 3042(T), isolated from a deep-sea brittle star in the Fiji Sea, was subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed marine Mn(II)-oxidizing isolate S185-2B as the closest neighbour of strain KMM 3042(T) (99.9 % sequence similarity). The two strains formed a distinct lineage within the genus Pseudomonas adjacent to the members of the Pseudomonas borbori cluster, sharing highest sequence similarity of 97.4 and 97.0 %, respectively, with P. borbori DSM 17834(T) and Pseudomonas flavescens DSM 12071(T). The DNA-DNA hybridization value (71 %) between strains KMM 3042(T) and S185-2B confirmed their assignment to the same species. On the basis of phylogenetic analysis, DNA-DNA hybridization and physiological and biochemical characterization, strains KMM 3042(T) and S185-2B should be assigned to a novel species of the genus Pseudomonas, for which the name Pseudomonas marincola sp. nov. is proposed. The type strain is KMM 3042(T) (=NRIC 0729(T) =JCM 14761(T)).
从斐济海的深海海蛇尾中分离出的一株需氧、革兰氏阴性、具运动性、无色素的细菌KMM 3042(T)，进行了多相分类研究。基于16S rRNA基因序列的系统发育分析表明，海洋锰（II）氧化分离株S185-2B是菌株KMM 3042(T)最接近的亲缘种（序列相似性为99.9%）。这两个菌株在假单胞菌属中形成了一个与波氏假单胞菌簇成员相邻的独特谱系，分别与波氏假单胞菌DSM 17834(T)和荧光假单胞菌DSM 12071(T)的序列相似性最高，为97.4%和97.0%。菌株KMM 3042(T)和S185-2B之间的DNA-DNA杂交值（71%）证实它们属于同一物种。基于系统发育分析、DNA-DNA杂交以及生理生化特征，菌株KMM 3042(T)和S185-2B应归为假单胞菌属的一个新物种，为此提出新名称海洋假单胞菌（Pseudomonas marincola sp. nov.）。模式菌株为KMM 3042(T)（=NRIC 0729(T) =JCM 14761(T)）。
