木糖氧化无色杆菌天然存在的D类β-内酰胺酶的特性分析
Characterization of a naturally occurring class D beta-lactamase from Achromobacter xylosoxidans.
作者信息
Doi Yohei, Poirel Laurent, Paterson David L, Nordmann Patrice
机构信息
Service de Bactériologie-Virologie, INSERM U914, Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté deMédecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France.
出版信息
Antimicrob Agents Chemother. 2008 Jun;52(6):1952-6. doi: 10.1128/AAC.01463-07. Epub 2008 Mar 24.
Abstract
A chromosomally encoded class D beta-lactamase, OXA-114, was characterized from Achromobacter xylosoxidans strain CIP69598. beta-Lactamase OXA-114 shared 56% amino acid identity with the naturally occurring class D beta-lactamase of Burkholderia cenocepacia and 42% identity with the acquired oxacillinases OXA-9 and OXA-18. OXA-114 has a narrow-spectrum hydrolysis profile, although it includes imipenem, at a very low level. PCR and sequencing revealed that bla(OXA-114)-like genes were identified in all A. xylosoxidans strains tested (n = 5), indicating that this beta-lactamase is naturally occurring in that species. Induction experiments with imipenem and cefoxitin did not show inducibility of bla(OXA-114) expression.
摘要
从木糖氧化无色杆菌CIP69598菌株中鉴定出一种染色体编码的D类β-内酰胺酶OXA-114。β-内酰胺酶OXA-114与洋葱伯克霍尔德菌天然存在的D类β-内酰胺酶有56%的氨基酸同一性，与获得性苯唑西林酶OXA-9和OXA-18有42%的同一性。OXA-114具有窄谱水解谱，尽管它包括亚胺培南，但水解水平非常低。PCR和测序显示，在所测试的所有木糖氧化无色杆菌菌株（n = 5）中均鉴定出bla(OXA-114)样基因，这表明该β-内酰胺酶在该菌种中天然存在。用亚胺培南和头孢西丁进行的诱导实验未显示bla(OXA-114)表达的可诱导性。
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