Gershon A A, Steinberg S, Smith M
Infect Immun. 1976 Jun;13(6):1549-53. doi: 10.1128/iai.13.6.1549-1553.1976.
Cell-mediated immunity to varicella-zoster (V-Z) virus in persons immune to varicella has been demonstrated, using a tissue culture technique. Cell-mediated immunity was reflected by the ability of peripheral leukocytes (lymphocytes and monocytes) from human donors to inactivate V-Z virus. Leukocytes were stimulated by the addition of noninfectious V-Z antigen to cultures newly infected with V-Z virus. Several days leter, the V-Z virus in these cultures was titered. When leukocytes from donors immune to caricella were used, a significant decrease in V-Z titer, compared with controls, was noted. When leukocytes from donors susceptible to varicella were tested, no decrease in V-Z virus titer was found. A mixed population of lymphocytes and monocytes from immune donors was required to demonstrate inactivation of V-Z virus. The development of specific cell-mediated immunity to V-Z virus may play a role in termination of varicella and in prevention of second attacks of this disease.
利用组织培养技术已证实，对水痘有免疫力的人对水痘 - 带状疱疹（V - Z）病毒存在细胞介导免疫。细胞介导免疫通过人类供体的外周白细胞（淋巴细胞和单核细胞）使V - Z病毒失活的能力得以体现。通过向新感染V - Z病毒的培养物中添加非感染性V - Z抗原刺激白细胞。几天后，对这些培养物中的V - Z病毒进行滴定。当使用对水痘有免疫力的供体的白细胞时，与对照组相比，V - Z滴度显著降低。当检测对水痘易感的供体的白细胞时，未发现V - Z病毒滴度降低。需要来自免疫供体的淋巴细胞和单核细胞混合群体才能证明V - Z病毒的失活。对V - Z病毒特异性细胞介导免疫的发展可能在水痘的终止以及预防该疾病的再次发作中起作用。
