由干扰素介导的对单纯疱疹病毒的细胞免疫。
Cellular immunity to herpes simplex virus mediated by interferon.
作者信息
Lodmell D L, Notkins A L
出版信息
J Exp Med. 1974 Sep 1;140(3):764-78. doi: 10.1084/jem.140.3.764.
Rabbit kidney cell monolayers infected with herpes simplex virus (HSV) were incubated with leukocytes from rabbits immunized with complete Freund's adjuvant. When the leukocytes were exposed to tuberculin purified protein derivative (PPD), viral replication and plaque formation were markedly inhibited. Similarly, when leukocytes from animals immunized with HSV were exposed to UV-inactivated HSV, viral replication was markedly inhibited. Exposure of leukocytes from unimmunized animals or animals immunized with incomplete Freund's adjuvant to UV-inactivated virus or PPD produced relatively little inhibition of viral replication. Examination of supernatant fluids from stimulated cultures revealed a soluble mediator that had the properties of interferon. Interferon production was detected within several hours after exposure of sensitized leukocytes to antigen. Supernatant fluids from as few as one sensitized leukocyte per 200 rabbit kidney cells inhibited HSV replication by over 90%. These findings support the concept that the cellular immune response to HSV consists of two phases: an immunologically specific antigen recognition phase, and a nonspecific effector phase that stops HSV spread by generating interferon.
用单纯疱疹病毒（HSV）感染兔肾细胞单层，然后与用完全弗氏佐剂免疫的兔的白细胞一起孵育。当白细胞暴露于结核菌素纯化蛋白衍生物（PPD）时，病毒复制和噬斑形成受到明显抑制。同样，当用HSV免疫的动物的白细胞暴露于紫外线灭活的HSV时，病毒复制也受到明显抑制。将未免疫动物或用不完全弗氏佐剂免疫的动物的白细胞暴露于紫外线灭活的病毒或PPD，对病毒复制的抑制作用相对较小。对刺激培养物的上清液进行检测，发现了一种具有干扰素特性的可溶性介质。在致敏白细胞暴露于抗原后的数小时内即可检测到干扰素的产生。每200个兔肾细胞中只要有一个致敏白细胞的上清液就能使HSV复制受到90%以上的抑制。这些发现支持了这样一种观点，即对HSV的细胞免疫反应包括两个阶段：免疫特异性抗原识别阶段，以及通过产生干扰素来阻止HSV传播的非特异性效应阶段。
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