Department of Integrative Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Chin Med. 2008 Apr 29;3:5. doi: 10.1186/1749-8546-3-5.
Dachengqi Tang (DT) is a common traditional Chinese medicine formula for expelling neire ('internal heat') in the stomach and intestines. There was no reliable analytical method available for the quality control of DT.
A high-performance liquid chromatography (HPLC) method with a reverse phase C18 column (150 x 4.6 mm) was developed. The mobile phase was methanol with 0.2% acetic acid. Eight markers including naringin, hesperidin, aloe emodin, rhein, honokiol, magnolol, emodin and chrysophanol were determined.
Regression analysis revealed a linear relationship between the concentrations of the markers and the peak area ratio of the standards and internal standard. The limit of detection (S/N = 3) and the limit of qualification (RSD < 20%) ranged from 0.21 to 0.43 ng/microl and 0.76 to 1.74 ng/microl respectively. The recovery was between 95.6% and 103.4%. The tests on the samples from three batches of DT showed that the profiles of the markers did not vary significantly among batches.
A reliable HPLC method for simultaneous determination of the eight markers in DT was developed.
大承气汤（DT）是一种常见的中药方剂，用于驱除胃和肠道中的“内热”。目前还没有可靠的分析方法来控制 DT 的质量。
建立了一种高效液相色谱（HPLC）方法，使用反相 C18 柱（150×4.6mm）。流动相为甲醇加 0.2%乙酸。测定了 8 种标志物，包括柚皮苷、橙皮苷、芦荟大黄素、大黄酸、和厚朴酚、厚朴酚、大黄素和大黄酚。
回归分析显示，标志物的浓度与标准品和内标物的峰面积比之间存在线性关系。检测限（S/N=3）和定量限（RSD<20%）范围分别为 0.21 至 0.43ng/微升和 0.76 至 1.74ng/微升。回收率在 95.6%至 103.4%之间。对三批 DT 样品的测试表明，这些批次的标志物图谱没有明显差异。
建立了一种可靠的 HPLC 方法，可同时测定 DT 中的 8 种标志物。
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