Minamida Kimiko, Ota Kyohei, Nishimukai Megumi, Tanaka Michiko, Abe Ayumi, Sone Teruo, Tomita Fusao, Hara Hiroshi, Asano Kozo
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
Int J Syst Evol Microbiol. 2008 May;58(Pt 5):1238-40. doi: 10.1099/ijs.0.64894-0.
An obligately anaerobic and equol-producing bacterium, designated strain do03T, was isolated from the caecal content of a rat. Cells were Gram-positive, non-spore-forming rods. The results from a phylogenetic analysis based on 16S rRNA gene sequences showed that strain do03T formed a separate line of descent in the phylogenetic cluster of the family Coriobacteriaceae. The strain was unable to metabolize glucose or other carbohydrates as sole carbon sources; growth was enhanced in the presence of arginine. The cell wall contained meso-diaminopimelic acid. The major fatty acid was C18 : 1cis9 (54.0 %). The strain had one unidentified predominant (91.9 %) quinone that was not menaquinone, methylmenaquinone, demethylmenaquinone, ubiquinone or rhodoquinone. The DNA G+C content was 63 mol%. The data presented in this work show that strain do03T differs from members of the related recognized genera Eggerthella and Denitrobacterium at both the phylogenetic and phenotypic level. Therefore, the strain constitutes a novel genus and species, for which the name Asaccharobacter celatus gen. nov., sp. nov. is proposed. The type strain of the type species is do03T (=JCM 14811T=DSM 18785T=AHU 1763T).
从一只大鼠的盲肠内容物中分离出一株专性厌氧且能产生雌马酚的细菌，命名为do03T菌株。细胞为革兰氏阳性、不产芽孢的杆菌。基于16S rRNA基因序列的系统发育分析结果表明，do03T菌株在科里杆菌科的系统发育簇中形成了一个独立的谱系。该菌株不能以葡萄糖或其他碳水化合物作为唯一碳源进行代谢；在精氨酸存在的情况下生长增强。细胞壁含有内消旋二氨基庚二酸。主要脂肪酸为C18 : 1cis9（54.0%）。该菌株有一种未鉴定的主要醌（91.9%），不是甲萘醌、甲基萘醌、去甲基萘醌、泛醌或视黄醌。DNA的G+C含量为63 mol%。这项工作中提供的数据表明，do03T菌株在系统发育和表型水平上均与相关的已确认属埃格特菌属和脱氮杆菌属的成员不同。因此，该菌株构成了一个新的属和种，为此提出新属名为Asaccharobacter，新种名为celatus gen. nov., sp. nov.。模式种的模式菌株为do03T（=JCM 14811T=DSM 18785T=AHU 1763T）。
