Martin J R, Holt R K, Langston C, Gilden D H, Richardson E P, Manz H J, Singer D B
Laboratory of Experimental Neuropathology, National Institutes of Health, Bethesda, MD 20892.
Hum Pathol. 1991 Jan;22(1):75-80. doi: 10.1016/0046-8177(91)90065-w.
To identify antigens of herpes simplex virus (HSV) types 1 and 2 and varicella-zoster virus (VZV) in human tissue, polyclonal antisera and an immunoperoxidase method were used to examine formalin-fixed, paraffin-embedded tissues from autopsy cases and experimentally infected animals. These antisera readily distinguished between HSV and VZV antigen, with no evident cross-reactivity. Antiser ato HSV-1 and HSV-2 were more strongly reactive with antigen of the homologous virus than with that of heterologous virus. This difference in immunoreactivity was used to discriminate between HSV-1 and HSV-2 antigens in experimentally infected animal tissues containing HSV antigens of known type and, by extrapolation, to distinguish between these antigens in human autopsy tissues. Thus, with appropriate antisera and tissue controls, HSV-1, HSV-2, and VZV can be identified in paraffin sections.
为了在人体组织中鉴定单纯疱疹病毒1型和2型（HSV-1和HSV-2）以及水痘-带状疱疹病毒（VZV）的抗原，采用多克隆抗血清和免疫过氧化物酶方法，对尸检病例和实验感染动物的福尔马林固定、石蜡包埋组织进行检查。这些抗血清能够轻易区分HSV和VZV抗原，无明显交叉反应。抗HSV-1和HSV-2血清与同源病毒抗原的反应比与异源病毒抗原的反应更强。这种免疫反应性的差异被用于在含有已知类型HSV抗原的实验感染动物组织中区分HSV-1和HSV-2抗原，并据此推断在人体尸检组织中区分这些抗原。因此，使用适当的抗血清和组织对照，可在石蜡切片中鉴定HSV-1、HSV-2和VZV。
