Vreeswijk J, Folkers E, Wagenaar F, Kapsenberg J G
Central Veterinary Institute, Department of Virology, Lelystad, The Netherlands.
J Virol Methods. 1988 Dec;22(2-3):255-71. doi: 10.1016/0166-0934(88)90108-5.
Colloidal gold immunoelectron microscopy was used to diagnose rapidly 53 cases clinically suspected of varicella-zoster virus (VZV) infection and one special case selected from another study on typical herpes simplex virus (HSV) infections. The viruses were identified and subsequently typed within 2.5 h by a direct labelling test for VZV, and within 3.5 h by an indirect labelling test with monoclonal antibodies against HSV type 1 and type 2. The protein A purified IgG fraction of human anti-VZV immunoglobulins was adsorbed to colloidal gold particles, and the specificity of the gold-labelled antibodies was tested with several human and animal herpesviruses. Viral envelopes did not crossreact in the direct labelling test. However, an indirect labelling procedure revealed that a small fraction of the anti-VZV antibodies crossreacted with the cores of herpes simplex virus and pseudorabies virus (Aujeszky disease virus). Virus-infected cellular material taken from typical herpetic lesions was used directly without virus propagation for virus typing. All cases (N = 54) were analyzed without knowing the clinical description of the results of cytopathologic examination (Tzanck smear) and viral culture. Forty-four cases were identified as VZV; however, 5 of the supposed VZV infections were proved to be HSV infections. Although the viral culture of the one HSV case was HSV- and VZV-negative, colloidal gold labelling identified the case as VZV infection. In 16 cases virus immunoglobulin complexes were detected by using gold-tagged antibodies against human immunoglobulins. Immunoglobulins on the viral envelopes did not interfere with virus typing by immunogold labelling.
采用胶体金免疫电子显微镜技术对53例临床疑似水痘-带状疱疹病毒（VZV）感染的病例以及从另一项关于典型单纯疱疹病毒（HSV）感染的研究中选取的1例特殊病例进行快速诊断。通过VZV直接标记试验在2.5小时内鉴定病毒并随后分型，通过针对1型和2型HSV的单克隆抗体间接标记试验在3.5小时内完成。将人抗VZV免疫球蛋白的蛋白A纯化IgG组分吸附到胶体金颗粒上，并用几种人和动物疱疹病毒检测金标记抗体的特异性。在直接标记试验中病毒包膜无交叉反应。然而，间接标记程序显示一小部分抗VZV抗体与单纯疱疹病毒和伪狂犬病病毒（奥耶斯基病病毒）的核心有交叉反应。取自典型疱疹性病变的病毒感染细胞材料直接用于病毒分型，无需病毒增殖。所有病例（N = 54）在不知道细胞病理学检查（Tzanck涂片）和病毒培养结果的临床描述的情况下进行分析。44例被鉴定为VZV；然而，5例疑似VZV感染被证实为HSV感染。尽管1例HSV病例的病毒培养为HSV和VZV阴性，但胶体金标记将该病例鉴定为VZV感染。在16例病例中，通过使用抗人免疫球蛋白的金标记抗体检测到病毒免疫球蛋白复合物。病毒包膜上的免疫球蛋白不干扰免疫金标记进行病毒分型。
