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通过聚合酶链反应检测水痘-带状疱疹病毒（VZV）患者临床样本中的VZV DNA。

Detection of varicella-zoster virus (VZV) DNA in clinical samples from patients with VZV by the polymerase chain reaction.

作者信息

Kido S, Ozaki T, Asada H, Higashi K, Kondo K, Hayakawa Y, Morishima T, Takahashi M, Yamanishi K

机构信息

Department of Virology, Osaka University, Japan.

出版信息

J Clin Microbiol. 1991 Jan;29(1):76-9. doi: 10.1128/jcm.29.1.76-79.1991.

DOI:10.1128/jcm.29.1.76-79.1991
PMID:1847154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269706/
Abstract

A polymerase chain reaction system for the detection of varicella-zoster virus was established. Of 25 nucleotides, 4 oligonucleotide pairs (regions of thymidine kinase, thymidylate synthetase, glycoprotein I, and immediate early gene) were synthesized. The first three oligonucleotide pairs could be used as primers on the basis of specific DNA amplification. Varicella-zoster virus DNA was amplified by this polymerase chain reaction system in 20 of 20 vesicle samples, 5 of 6 crusts, and 12 of 13 throat swabs collected from patients with clinical varicella.

摘要

建立了一种用于检测水痘-带状疱疹病毒的聚合酶链反应系统。在25个核苷酸中，合成了4对寡核苷酸（胸苷激酶区、胸苷酸合成酶区、糖蛋白I区和立即早期基因区）。基于特异性DNA扩增，前三对寡核苷酸可作为引物。通过该聚合酶链反应系统，在从临床水痘患者采集的20份水疱样本中的20份、6份痂皮样本中的5份以及13份咽拭子样本中的12份中扩增出了水痘-带状疱疹病毒DNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/6ac99c7453c5/jcm00037-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/78ef9cff6ef8/jcm00037-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/b48411fa8810/jcm00037-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/6ac99c7453c5/jcm00037-0100-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/78ef9cff6ef8/jcm00037-0099-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/b48411fa8810/jcm00037-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8bf7/269706/6ac99c7453c5/jcm00037-0100-b.jpg

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本文引用的文献

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Viremic phase in nonimmunocompromised children with varicella.非免疫功能低下的水痘患儿的病毒血症期
J Pediatr. 1984 Jan;104(1):85-7. doi: 10.1016/s0022-3476(84)80596-x.
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Isolation of varicella-zoster virus from pharyngeal and nasal swabs in varicella patients.从水痘患者的咽拭子和鼻拭子中分离水痘带状疱疹病毒。
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Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.通过聚合酶链反应生成单链DNA及其在HLA - DQA基因座直接测序中的应用。
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Identification of cytomegalovirus and human herpesvirus-6 DNA in a patient with corneal endotheliitis.鉴定角膜内皮炎患者的巨细胞病毒和人类疱疹病毒-6 DNA。
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