通过聚合酶链反应检测水痘-带状疱疹病毒（VZV）患者临床样本中的VZV DNA。
Detection of varicella-zoster virus (VZV) DNA in clinical samples from patients with VZV by the polymerase chain reaction.
作者信息
Kido S, Ozaki T, Asada H, Higashi K, Kondo K, Hayakawa Y, Morishima T, Takahashi M, Yamanishi K
机构信息
Department of Virology, Osaka University, Japan.
出版信息
J Clin Microbiol. 1991 Jan;29(1):76-9. doi: 10.1128/jcm.29.1.76-79.1991.
A polymerase chain reaction system for the detection of varicella-zoster virus was established. Of 25 nucleotides, 4 oligonucleotide pairs (regions of thymidine kinase, thymidylate synthetase, glycoprotein I, and immediate early gene) were synthesized. The first three oligonucleotide pairs could be used as primers on the basis of specific DNA amplification. Varicella-zoster virus DNA was amplified by this polymerase chain reaction system in 20 of 20 vesicle samples, 5 of 6 crusts, and 12 of 13 throat swabs collected from patients with clinical varicella.
建立了一种用于检测水痘-带状疱疹病毒的聚合酶链反应系统。在25个核苷酸中，合成了4对寡核苷酸（胸苷激酶区、胸苷酸合成酶区、糖蛋白I区和立即早期基因区）。基于特异性DNA扩增，前三对寡核苷酸可作为引物。通过该聚合酶链反应系统，在从临床水痘患者采集的20份水疱样本中的20份、6份痂皮样本中的5份以及13份咽拭子样本中的12份中扩增出了水痘-带状疱疹病毒DNA。
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