直接免疫荧光法和病毒培养法在检测皮肤病变中水痘-带状疱疹病毒的效用。
Utility of direct immunofluorescence and virus culture for detection of varicella-zoster virus in skin lesions.
作者信息
Coffin S E, Hodinka R L
机构信息
Department of Pediatrics, Children's Hospital of Philadelphia and School of Medicine, University of Pennsylvania 19104, USA.
出版信息
J Clin Microbiol. 1995 Oct;33(10):2792-5. doi: 10.1128/jcm.33.10.2792-2795.1995.
Abstract
A direct immunofluorescence assay (DFA) with a monoclonal antibody from Ortho Diagnostic Systems was compared with conventional cell culture for the rapid detection of varicella-zoster virus (VZV) in 140 dermal lesions from 133 patients. A total of 79 (56%) specimens were positive for VZV: 40 (51%) by DFA alone, 2 (3%) by culture only, and 37 (47%) by both culture and DFA. After discordant analysis, the sensitivities and negative predictive values, respectively, were 97.5% (77 of 79) and 96.8% (61 of 63) for DFA and 49.4% (39 of 79) and 60.4% (61 of 101) for viral culture. Of the 39 positive viral cultures, VZV was isolated from 38 (97%) cultures in A549 cells, 23 (59%) in primary rhesus monkey kidney cells, and only 16 (41%) in MRC-5 cells. We conclude that DFA is the optimal method for rapid identification of VZV. In addition, better recovery of VZV in culture may be achieved by using A549 cells.
摘要
将来自Ortho诊断系统的单克隆抗体直接免疫荧光测定法（DFA）与传统细胞培养法进行比较，以快速检测133例患者的140处皮肤损伤中的水痘带状疱疹病毒（VZV）。共有79份（56%）标本VZV呈阳性：仅DFA检测阳性的有40份（51%），仅培养检测阳性的有2份（3%），培养和DFA检测均阳性的有37份（47%）。经过不一致性分析，DFA的敏感性和阴性预测值分别为97.5%（79份中的77份）和96.8%（63份中的61份），病毒培养的敏感性和阴性预测值分别为49.4%（79份中的39份）和60.4%（101份中的61份）。在39份病毒培养阳性标本中，38份（97%）在A549细胞中分离出VZV，23份（59%）在原代恒河猴肾细胞中分离出VZV，仅16份（41%）在MRC-5细胞中分离出VZV。我们得出结论，DFA是快速鉴定VZV的最佳方法。此外，使用A549细胞可能会在培养中更好地回收VZV。
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