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两株室内空气来源的曲霉菌黑组新种

Two novel species of Aspergillus section Nigri from indoor air.

机构信息

EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, New Jersey 08077 USA;

出版信息

IMA Fungus. 2012 Dec;3(2):159-73. doi: 10.5598/imafungus.2012.03.02.08. Epub 2012 Nov 30.

DOI:10.5598/imafungus.2012.03.02.08
PMID:23355969
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3539319/
Abstract

Aspergillus floridensis and A. trinidadensis spp. nov. are described as novel uniseriate species of Aspergillus section Nigri isolated from air samples. To describe the species we used phenotypes from 7-d Czapek yeast extract agar culture (CYA), creatine agar culture (CREA) and malt extract agar culture (MEA), with support by molecular analysis of the β-tubulin, calmodulin, RNA polymerase II (RPB2), and translation elongation factor-alpha (TEF) gene amplified and sequenced from 56 air isolates and one isolate from almonds belonging to Aspergillus sectionNigri.Aspergillus floridensis is closely related to A. aculeatus, and A. trinidadensis is closely related to A. aculeatinus. Aspergillus brunneoviolaceus (syn. A. fijiensis) and A. uvarum are reported for the first time from the USA and from the indoor air environment. The newly described species do not produce ochratoxin A.

摘要

佛罗里达拟青霉和特立尼达拟青霉 spp. nov. 是从空气样本中分离出的青霉属黑组的新型单生种。为了描述这些物种,我们使用了来自 7 天 Czapek 酵母提取物琼脂培养物(CYA)、肌酸琼脂培养物(CREA)和麦芽提取物琼脂培养物(MEA)的表型,同时支持通过β-微管蛋白、钙调蛋白、RNA 聚合酶 II(RPB2)和翻译延伸因子-α(TEF)基因的分子分析,这些基因从 56 个空气分离物和一个来自杏仁的属于青霉属黑组的分离物中扩增和测序。佛罗里达拟青霉与棘孢青霉密切相关,特立尼达拟青霉与棘孢青霉 aculeatinus 密切相关。黑曲霉(syn. A. fijiensis)和黑曲霉首次报道来自美国和室内空气环境。新描述的物种不产生赭曲霉毒素 A。

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Two novel species of Aspergillus section Nigri from indoor air.两株室内空气来源的曲霉菌黑组新种
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β-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing and taxonomic consequences.β-微管蛋白同工型 tubC 在黑曲霉分类中常被错误鉴定为 benA 基因:引物特异性测试和分类学后果。
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