Singh Pratap B, Singh Vandana, Nayak P K
Department of Zoology, Tilak Dhari College, Jaunpur 222 002, India.
Food Chem Toxicol. 2008 Jul;46(7):2533-9. doi: 10.1016/j.fct.2008.04.009. Epub 2008 Apr 15.
Organochlorines (isomers of hexachlorocyclohexane--HCHs and metabolites of dichlorodiphenyltrichloroethane--DDTs, aldrin and endosulfan) and organophosphate (chlorpyrifos) insecticide residues were investigated by gas liquid chromatography in the blood of fish, chick, goat and man. The plasma levels of testosterone (T) and estradiol-17beta (E2) was measured by radioimmunoassay in the catfish Rita rita captured from unpolluted reference site and polluted river Gomti during prespawning phase. Results indicated that in R. rita the SigmaDDT, SigmaHCH, endosulfan, aldrin, chlorpyrifos in blood levels were in preferential order (SigmaDDT>SigmaHCH>endosulfan>aldrin>chlorpyrifos) of their bioaccumulation. The blood levels of SigmaHCH and SigmaDDT also showed high levels in chick, goat and man, and preferential order of bioaccumulation was goat>chick>man>fish. The SigmaDDT also showed preferential order (man>chick>goat>fish) of bioaccumulation. Among the different tissues of fish (blood, liver, brain and ovary) the SigmaDDT was very high as compared to SigmaHCH as well as the rest of tissues which was very selective bioconcentration in different tissues of fish during prespawning phase. The gonado-somatic index, T and E2 declined in the catfish captured from polluted river when compared with the catfish captured from reference site affecting reproductive physiology. Our results indicated that increase of insecticides in blood level in vertebrates causes reproductive dysfunction and suggested that for human beings food like fish, chick and goat containing beyond permissible limit of insecticides must be avoided.
采用气液色谱法对鱼类、雏鸡、山羊和人类血液中的有机氯（六氯环己烷异构体——六氯环己烷和二氯二苯三氯乙烷代谢物——滴滴涕、艾氏剂和硫丹）和有机磷（毒死蜱）杀虫剂残留进行了研究。在产卵前期，对从未受污染的参照点和受污染的贡蒂河捕获的鲇鱼丽塔鲇血浆中的睾酮（T）和雌二醇-17β（E2）水平进行了放射免疫测定。结果表明，在丽塔鲇中，血液中总滴滴涕、总六氯环己烷、硫丹、艾氏剂、毒死蜱的生物累积优先顺序为（总滴滴涕>总六氯环己烷>硫丹>艾氏剂>毒死蜱）。总六氯环己烷和总滴滴涕的血液水平在雏鸡、山羊和人类中也呈现高水平，生物累积的优先顺序为山羊>雏鸡>人类>鱼类。总滴滴涕的生物累积也呈现优先顺序（人类>雏鸡>山羊>鱼类）。在鱼类的不同组织（血液、肝脏、大脑和卵巢）中，总滴滴涕与总六氯环己烷以及其他组织相比非常高，这表明在产卵前期鱼类不同组织中存在非常有选择性的生物富集。与从参照点捕获的鲇鱼相比，从受污染河流捕获的鲇鱼的性腺-体指数、T和E2下降，影响了生殖生理。我们的结果表明，脊椎动物血液中杀虫剂水平的升高会导致生殖功能障碍，并建议人类必须避免食用含有超过杀虫剂允许限量的鱼类、雏鸡和山羊等食物。
