Doerig C, Pizer L I, Wilcox C L
Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
J Virol. 1991 May;65(5):2724-7. doi: 10.1128/JVI.65.5.2724-2727.1991.
During latent infection of neurons with herpes simplex virus type 1, viral transcription is restricted to the latency-associated transcripts (LATs). These RNAs contain open reading frames, but detection of a protein encoded by the LATs has not been reported. We used immunocytochemical techniques to demonstrate that an antiserum directed against a bacterially expressed fusion protein containing part of a LAT-encoded polypeptide recognized an antigen present in primary neurons latently infected in vitro. This antigen (called LAA, for latency-associated antigen) was not detected in mock-infected neurons, in productively infected Vero cells, or in neurons latently infected with a mutant virus carrying a deletion in the LAT gene. By Western immunoblot analysis, we demonstrated the presence of a protein with an apparent molecular mass of 80 kDa recognized by the anti-LAA antiserum in latently infected neurons.
在1型单纯疱疹病毒对神经元的潜伏感染过程中，病毒转录仅限于潜伏相关转录本（LATs）。这些RNA含有开放阅读框，但尚未有关于LATs编码蛋白的检测报道。我们使用免疫细胞化学技术证明，一种针对含有部分LAT编码多肽的细菌表达融合蛋白的抗血清，能够识别体外潜伏感染的原代神经元中存在的一种抗原。在模拟感染的神经元、产生性感染的Vero细胞或携带LAT基因缺失的突变病毒潜伏感染的神经元中，均未检测到这种抗原（称为LAA，即潜伏相关抗原）。通过蛋白质免疫印迹分析，我们证实在潜伏感染的神经元中存在一种表观分子量为80 kDa的蛋白，该蛋白可被抗LAA抗血清识别。
