Joosten M M, Beulens J W J, Kersten S, Hendriks H F J
Business Unit Biosciences, TNO Quality of Life, P. O. Box 360, 3700 AJ, Zeist, The Netherlands.
Diabetologia. 2008 Aug;51(8):1375-81. doi: 10.1007/s00125-008-1031-y. Epub 2008 May 27.
AIMS/HYPOTHESIS: To determine whether 6 weeks of daily, moderate alcohol consumption increases expression of the gene encoding adiponectin (ADIPOQ) and plasma levels of the protein, and improves insulin sensitivity in postmenopausal women.
In a randomised, open-label, crossover trial conducted in the Netherlands, 36 apparently healthy postmenopausal women who were habitual alcohol consumers, received 250 ml white wine ( approximately 25 g alcohol/day) or 250 ml of white grape juice (control) daily during dinner for 6 weeks. Randomisation to treatment allocation occurred according to BMI. Insulin sensitivity and ADIPOQ mRNA and plasma adiponectin levels were measured at the end of both periods. Insulin sensitivity was estimated using the homeostasis model assessment of insulin resistance (HOMA-IR). Levels of ADIPOQ mRNA in subcutaneous adipose tissue were determined by RT-PCR.
All subjects completed the study. Six weeks of white wine consumption reduced fasting insulin (mean +/- SEM 40.0 +/- 3.4 vs 46.5 +/- 3.4 pmol/l; p < 0.01) and HOMA-IR (1.42 +/- 0.13 vs 1.64 +/- 0.13; p = 0.02) compared with 6 weeks of grape juice consumption. ADIPOQ mRNA levels (1.09 +/- 0.15 vs 0.98 +/- 0.15; p = 0.04) and plasma levels of total (13.1 +/- 0.8 vs 12.0 +/- 0.8 microg/ml; p < 0.001) and high molecular weight (HMW) adiponectin (9.9 +/- 1.2 vs 8.8 +/- 1.2 microg/ml; p = 0.02) significantly increased after alcohol compared with juice consumption. Changes in ADIPOQ mRNA levels correlated with changes in plasma levels of total adiponectin (rho = 0.46; p < 0.01). Both fasting triacylglycerol (8.2%; p = 0.04) and LDL-cholesterol levels (7.8%; p < 0.0001) decreased, whereas HDL-cholesterol increased (7.0%; p < 0.0001) after prolonged moderate alcohol intake. No notable adverse effects were reported.
CONCLUSIONS/INTERPRETATION: Moderate alcohol consumption for 6 weeks improves insulin sensitivity, adiponectin levels and lipid profile in postmenopausal women. Furthermore, these data suggest a transcriptional mechanism leading to the alcohol-induced increase in adiponectin plasma levels.
目的/假设：确定绝经后女性每日适度饮酒6周是否会增加脂联素（ADIPOQ）编码基因的表达、血浆蛋白水平，并改善胰岛素敏感性。
在荷兰进行的一项随机、开放标签、交叉试验中，36名明显健康的绝经后习惯性饮酒女性在晚餐时每天饮用250毫升白葡萄酒（约25克酒精/天）或250毫升白葡萄汁（对照），持续6周。根据体重指数进行治疗分配随机化。在两个阶段结束时测量胰岛素敏感性、ADIPOQ mRNA和血浆脂联素水平。使用胰岛素抵抗稳态模型评估（HOMA-IR）估计胰岛素敏感性。通过RT-PCR测定皮下脂肪组织中ADIPOQ mRNA的水平。
所有受试者均完成研究。与饮用葡萄汁6周相比，饮用白葡萄酒6周可降低空腹胰岛素水平（平均±标准误 40.0±3.4 vs 46.5±3.4 pmol/l；p<0.01）和HOMA-IR（1.42±0.13 vs 1.64±0.13；p = 0.02）。与饮用葡萄汁相比，饮酒后ADIPOQ mRNA水平（1.09±0.15 vs 0.98±0.15；p = 0.04）以及总脂联素（13.1±0.8 vs 12.0±0.8 μg/ml；p<0.001）和高分子量（HMW）脂联素的血浆水平（9.9±1.2 vs 8.8±1.2 μg/ml；p = 0.02）显著升高。ADIPOQ mRNA水平的变化与总脂联素血浆水平的变化相关（rho = 0.46；p<0.01）。长期适度饮酒后，空腹甘油三酯（8.2%；p = 0.04）和低密度脂蛋白胆固醇水平（7.8%；p<0.0001）均下降，而高密度脂蛋白胆固醇升高（7.0%；p<0.0001）。未报告明显不良反应。
结论/解读：绝经后女性适度饮酒6周可改善胰岛素敏感性、脂联素水平和血脂谱。此外，这些数据提示了一种转录机制，导致酒精诱导的脂联素血浆水平升高。
