Tramuta C, Robino P, Nebbia P
Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci, 44, 10095 Grugliasco (TO), Italy.
Vet Res Commun. 2008 Aug;32(6):433-7. doi: 10.1007/s11259-008-9042-1. Epub 2008 May 29.
Detection and distribution of eae gene in forty-four attaching and effacing Escherichia coli (AEEC) strains of animal origin were investigated. Association of distinct intimin alleles with phylogenetic background were assessed among strains in comparison with different serogroups. Phylogenetic analysis showed that 31 EHEC/eae+ STEC strains belong to groups A, B1 and E, 13 EPEC strains segregated in B1 and B2. Moreover, group A possessed the eae gamma2/theta type, group B1 the eae beta1, eae kappa, eae zeta, and eae epsilon types, group B2 the eae alpha1, eae alpha2 and eae iota types, while the group E possessed the eae gamma1 type. The presence of numerous eae-types show that EPEC and EHEC/eae+ STEC tested have a high genetic homology within each phylogenetic group.
对44株动物源紧密黏附性大肠杆菌（AEEC）菌株中eae基因的检测与分布进行了研究。与不同血清群的菌株相比，评估了不同intimin等位基因与系统发育背景的关联。系统发育分析表明，31株肠出血性大肠杆菌/ eae阳性的肠毒素型大肠杆菌（STEC）菌株属于A、B1和E组，13株肠致病性大肠杆菌（EPEC）菌株分属于B1和B2组。此外，A组拥有eae gamma2 / theta型，B1组拥有eae beta1、eae kappa、eae zeta和eae epsilon型，B2组拥有eae alpha1、eae alpha2和eae iota型，而E组拥有eae gamma1型。众多eae型的存在表明，所检测的EPEC和肠出血性大肠杆菌/eae阳性的STEC在每个系统发育组内具有高度的遗传同源性。
